蝉拟青霉多糖对人外周血单个核细胞及白血病细胞株U937、K562增殖的调节作用  

The m odulating effects of PCPS on proliferative activity of mononuclear cell and leukemic cell line U937 and K562 in human peripheral blood

作　　者：陈柏坤[1] 杨介钻[2] 卓佳[1] 董海燕[2] 金丽琴[3] 

机构地区：[1]温州医学院生物实验教学中心,浙江温州325035 [2]温州医学院微生物学教研室,浙江温州325035 [3]温州医学院生物化学教研室,浙江温州325035

出　　处：《温州医学院学报》2006年第4期341-344,共4页Journal of Wenzhou Medical College

基　　金：浙江省教育厅科研基金资助项目(NO.20020471);温州市"551"人才基金资助项目。

摘　　要：目的:研究蝉拟青霉总多糖对人外周血单个核细胞抗肿瘤活性的促进作用及其可能对肿瘤细胞株U937、K562的直接抑制作用。方法:用水提取蝉拟青霉菌丝体总多糖,以MTT法测定不同浓度总多糖对人外周血单个核细胞及白血病细胞株U937、K562增殖的影响,ELISA法测定多糖作用44h后,外周血单个核细胞培养上清液中hTNF-α、hIFN-γ的含量。结果:一定浓度的蝉拟青霉多糖能使外周血单个核细胞的增殖率升高,较高浓度多糖能抑制白血病细胞株U937、K562的增殖,同时蝉拟青霉多糖能提高外周血单个核细胞表达hTNF-α、hIFN-γ。结论:蝉拟青霉多糖可能具有直接抑制U937、K562的增殖作用;能提高外周血单个核细胞的增殖能力,使其分泌hTNF-α、hIFN-γ升高,从而可能促进外周血单个核细胞的杀肿瘤活性。Objective： To investigate the effect of PCPS on the modulation of the anti-tumoral activity of human peripheral blood mononuclear celI（PBMC） and direct suppression of the proliferative activity of leukemic cell line U937 and K562. Methods： The total polysaccharide was extracted with distilled water from mycelia of paecilomyces cicadae. The colorimetric MTT was used to analyze the proliferative activity of PBMC, U937 and K562 which were treated with PCPS of 7 concentrations resDectively in vitro. ELISA was used to detect the content of hTNF-α, hIFN-γ from supernatant of culturing PBMC that was treated with PCPS for 44 h. Results： The proliferative rate of PBMC was increased when they were treated with PCPS. Otherwise the proliferative activity of U937 and K562 were suppressed by PCPS of certain concentration. While the PCPS can enhance the hTNF-α.hIFN-γ productions secrected by PBMC in vitro. Conclusions： The PCPS can directly suppress the proliferative activity of U937 and K562. and can enhance the proliferative activity of PBMC and the productions of hTNF-α. hIFN-γsecreted by PBMC, then probably improve anti-tumoral activity of PBMC.

关 键 词：蝉拟青霉多糖 外周血单个核细胞 白血病细胞 增殖活性 

分 类 号：R282[医药卫生—中药学]