混合二元醇改性处理对减轻戊二醛牛心包细胞毒性的作用  

Post-treated with polydiol reduced the cytotoxicity of glutaraldehyde tanned bovine pericardium

作　　者：吴国伟[1] 龚光甫[2] 黄柏英[3] 胡型锑[1] 王胜利[1] 杨美高[1] 

机构地区：[1]温州医学院第二附属医院心胸外科,浙江温州325027 [2]中南大学湘雅二院心胸外科,湖南长沙410000 [3]中南大学湘雅医学院细胞中心,湖南长沙410078

出　　处：《温州医学院学报》2006年第4期347-349,共3页Journal of Wenzhou Medical College

基　　金：温州市科技局科研基金资助项目(Y2005A151)。

摘　　要：目的:研究混合二元醇对减轻戊二醛牛心包细胞毒性的作用。方法:牛心包分两组——A组:用0.625%戊二醛固定2w后用0.3%戊二醛保存;B组:牛心包用0.625%戊二醛固定2w后,用混合二元醇后处理并保存。C组:玻片对照组。二组心包均切成2cm×2cm,分别种植同样数目的(4×104)人胚肺成纤维细胞,培养7d后,分别计算每片心包上活细胞数。同时作光镜及扫描电镜观察。结果:①活细胞计数结果:A组、B组与对照组相比差异均有显著性(P<0.01)。B组与A组的活细胞数差异有显著性(P<0.01)。②光镜下:对照组细胞生长良好,A、B组均有死亡细胞,尤以A组为多。③扫描电镜:A组活细胞很少,且多为圆球形细胞;B组有较多的细胞覆盖生长,可见细胞分裂相。结论:混合二元醇改性处理后的戊二醛牛心包细胞毒性明显降低。Objective：To research that post-treated with polydiol affect the cytotoxicity of glutaraldehyde tanned bovine pericardium （G-tBP）. Methods：Two groups of variouly preserved bovine pericardium tissue samples were used： Group A： Pericardium tanned with 0.625% glutaraldehyde（GA）for two weeks, then preserved in 0.3% GA; Group B： treated as group A, then post-treated with polydiol for two months; Group C： Glass was used as a control. Each group was 6 specimens. The tissue was cut into same size （2cm×2cm）. Same amount （4×10^4） of human embryo lung （HEL） fibroblast were seeding on the smooth surface of pericardium and glass sheet. After cultured for 7 days, living cells on each specimens were counted, and light microscopy and scanning electron microscopy （SEM） was examined. Result： The amount of living cells ：Group A vsGroup B, P〈0.01; Group C vs Group A、B, P〈0.01. Light microscopy showed the cells of group C were growing well. There were some dead cells in other groups, especially in group A. SEM showed there were only few cells on the surface of pericardiom in groupA. There were much more cells in group B. Conclusion： After glutaraldehyde tanned bovine pericardium post-treated with polydiol, its cytotoxicity reduces significantly.

关 键 词：混合醇 牛心包 戊二醛 细胞毒性 

分 类 号：R318.08[医药卫生—生物医学工程]