超抗原金黄色葡萄球菌肠毒素A对T淋巴细胞分化的作用  

Effects of Superantigen SEA on the Differentiation of T-lymphocytes

作　　者：邓晓芳[1] 曾波航[2] 胡伟民[1] 陈静琦[2] 黄慧[2] 孙建聪[2] 彭燕[3] 李冰[3] 

机构地区：[1]广州医学院附属肿瘤医院,广东广州510095 [2]广州医学院第二附属医院肿瘤科,广东广州510260 [3]广州医学院分子实验中心,广东广州510182

出　　处：《广州医学院学报》2006年第3期6-8,共3页Academic Journal of Guangzhou Medical College

基　　金：广东省科学技术厅基金项目(2004B31201013)

摘　　要：目的:探讨超抗原金黄色葡萄球菌肠毒素A(stapylococoal enterotoxin A,SEA)对T淋巴细胞亚群Tc/Th细胞增殖的不同影响。方法:以SEA刺激脐带血单个核细胞(CBMCs),以3H-TdR掺入法及ELISA法检测Tc/Th细胞对SEA刺激的反应。结果:经SEA刺激后,CBMCs中的Tc0/Th0增殖并向Ⅰ型或Ⅱ型分化。在较低浓度SEA刺激时,CBMCs分泌IL-10的比例高于γ-IFN;而经较高浓度SEA刺激后,CBMC分泌γ-IFN的比例高于IL-10。结论:SEA在较低浓度刺激时,诱导T淋巴细胞向Tc1/Th1的分化;而较高浓度时,在促进T细胞增殖的同时,可以诱导T细胞向Tc2/Th2的分化。Objective：To investigate the influence of the superantigen Staphylococcus enterotoxin A （SEA） on the differentiation of T lymphocyte. Methods： The cord blood mononuclear cells （CBMCs） were stimulated by SEA, and the response of Tc/Th in CBMCs to SEA stimulation was determined by ^3H-TdR incorporation and ELISA. Results： SEA induced Tc0/Th0 in CBMC proliferation and differentiation into type Ⅰ or Ⅱ T cells. The T cell secreted higher percentage of IL-10 than of γ-IFN with low-level SEA stimulation, while higher percentage of γ-IFN than of IL-10 with high-level SEA stimulation. Conclusion： T lymphocytes differentiated into Tc1/Th1 when stimulated by low-level SEA, but showed enhancement in proliferation and Tc2/Th2 differentiation when stimulated by high-level SEA.

关 键 词：金黄色葡萄球菌肠毒素A 超抗原 T淋巴细胞 细胞分化 胎儿脐带血 体外实验 

分 类 号：R392.12[医药卫生—免疫学]