组织培养技术筛选香石竹低玻璃化无性系初报  

In Vitro Selection for Carnation Clone Against Vitrification

作　　者：黄宇翔[1] 吴祖建 柯昉[1] 刘金燕[1] 

机构地区：[1]福建省农业科学院花卉研究中心,福州350003 [2]福建省农林大学植物病毒研究所,福州350000

出　　处：《中国农学通报》2006年第8期88-90,共3页Chinese Agricultural Science Bulletin

基　　金：福建省林业厅科技攻关项目"香石竹优质种苗繁育技术研究"(闽林<2004>科函9号)。

摘　　要：采用离体培养的方法,对香石竹红色品种‘多明格’的茎尖培养试管苗进行连续多代剔除玻璃苗和畸形苗的筛选,继代培养27代后获得了玻璃化率稳定在8%左右的低玻璃化无性系。该无性系经10代的增殖培养,定植到大田后仍然保持母本的优良性状,无明显的有害变异。Plantlets in vitro of ‘Domingo＇, one of the red-flowering carnation breeds, were subeultured in vitro. They were selected for obtaining a done, whose vitrification rate could be controlled less than 10%, by way of progressive subculture. After 27 times of subculture, such a clone was obtained. After 10 times of multiplying culture, the clone could keep the traits of its original one, and no harmful variation was observed, when they grew in a field.

关 键 词：香石竹 组织培养 玻璃化 无性系 

分 类 号：S68[农业科学—观赏园艺] Q813[农业科学—园艺学]