SARS冠状病毒S蛋白受体结合域的克隆测序  

Cloning and sequence analysis of receptor binding domain on the SARS-CoV spike protein

作　　者：高俊[1] 肖洪广[1] 林勇平[1] 

机构地区：[1]广州医学院第一附属医院检验科,广州510120

出　　处：《广州医药》2006年第5期49-52,共4页Guangzhou Medical Journal

基　　金：广州市科技局应用基础项目(NO2004J1-C0211);广州市教委科研项目(1040;1047)

摘　　要：目的从来源于人和果子狸的SARS冠状病毒S蛋白基因中,获得受体结合域(RBD)基因的克隆。方法用PCR方法扩增RBD基因,将其克隆到pGEM-T Easy载体,转化后挑取阳性克隆进行酶切和测序鉴定,并分析RBD基因序列。结果获得了人和果子狸来源的RBD的基因片段,长度为579 bp,两者具有高度的同源性。结果RBD是SARS冠状病毒与靶细胞结合的部位,本工作成功克隆了SARS冠状病毒的RBD基因,为该基因的表达和功能研究奠定了基础。Objective To sequence and analyze receptor binding domain （RBD） fragment on SARS-CoV Spike Protein form humans and palm civet. Methods RBD genes were amplified by PCR assay, then RBD segments were identified with restriction enzyme digestion and sequence after beening ligased into pGEM-T Easy vector. Results The RBD genes from two species were successfully cloned which were 579bp in length and high homologous. Conclusion The clone of RBD gene provide the basis of further study on expression and function research of RBD.

关 键 词：SARS冠状病毒 受体结合域 克隆 测序 

分 类 号：R346[医药卫生—基础医学]