毛细管电泳测定甲芬那酸制剂及体液中的甲芬那酸  

Determination of Mefenamic Acid in Mefenamic Acid Curatorial Products and Body Fluid by Capillary Electrophoresis

作　　者：林伟丰[1] 杨琳[1] 陈缵光[2] 李全文[2] 蔡沛祥[1] 莫金垣[1] 

机构地区：[1]中山大学化学与化学工程学院 [2]中山大学药学院,广州510089

出　　处：《分析科学学报》2006年第4期381-384,共4页Journal of Analytical Science

基　　金：广东省自然科学基金重点项目(No.021808);广东省教育厅科学基金(Z03055)

摘　　要：建立了制剂和体液中甲芬那酸毛细管电泳高频电导分析法，并用于甲芬那酸胶囊及血清、尿液中甲芬那酸含量的测定。对电泳介质的种类、浓度以及操作电压和进样量等影响因素进行了优化。实验采用3．0mmol／L环己胺十3．0mmol／LH3BO3十5．0mmol／Lβ-CD＋0．17mol／L乙醇作为电泳介质，20．0kV为分离电压，可在7min内实现对甲芬那酸的分离检测。在优化实验条件下，测定甲芬那酸的线性范围为0．8～550μg／mL，检出限为0．1μg／mL，回收率范围为98．3％～101．0％。A method based on capillary electrophoresis with high frequency conductivity detection （contactless conductivity detection） was developed for determination of mefenamic acid in mefenamic acid curatorial products and body fluid. In this study,major factors for separation and determinatign were studied and the optimal analysis conditions were obtained. In the buffer system of 3.0 mmol/L cyclohexylamine ±3.0 mmol/L boric acid ± 5. 0 mmol/L β-cyclodextrin ± 0. 17 mol/L ethanol and 20.0 kV of separation potential,mefenamic acid was separated and detected well within 7 min. The linear concentration ranged from 0.8 to 550μg/mL. The limit of detection reached 0. 1μg/mL. This method was used for analysis of mefenamic acid satisfactorily with a recovery of 98.1 -101%.

关 键 词：毛细管电泳 高频电导检测 非接触式电导检测 药物 甲芬那酸 体液 

分 类 号：O657.8[理学—分析化学] O657.1[理学—化学]