硝基苯酚位置异构体的毛细管电泳-方波安培检测研究  

Determination of Positional Isomers of Nitrophenol by Capillary Electrophoresis with Square-wave Amperometric Detection

作　　者：刘绮文[1] 谢天尧[1] 曾暖茜[1] 刘秋英[1] 

机构地区：[1]中山大学化学与化学工程学院,广州510275

出　　处：《分析科学学报》2006年第4期418-420,共3页Journal of Analytical Science

基　　金：广东省自然科学基金(No.031589)

摘　　要：在未涂层熔融石英毛细管（45cm×50μm i．d．）中，以pH-7．9的2mmol／L磷酸氢二钠＋0．4mmol／L磷酸二氢钠＋2mmol／Lβ-CD为电泳运行液，采用毛细管电泳-方波安培检测法，分离电压为＋11kV，可实现硝基苯酚3个位置异构体在6min内基线分离检测。方法的检出限为0．2～0．6mg／L。探讨了电泳运行液的组成、浓度、pH值、β-CD等因素对分离检测效果的影响。A rapid, sensitive and robust method of separation and determination of three positional isomers of nitrophenol by capillary electrophoresis with square-wave amperometric detection （SWAD） was developed. The positional isomers of nitrophenol were baseline separated within 6 min under the following separation conditions：2 mmol/L Na2HPO4 ＋0.4 mmol/L NaH2PO4 ＋ 2 mmol/L β-CD（pH = 7.9）as the running buffer, ＋11 kV as the separation voltage and Eu=1. 0 V as the square wave balance potential. The effects of the component and pH of the running buffer, the concentration of β-CD in buffer solution were discussed in detail. The calibration curves of m,p,and o-nitrophenol showed good linearity in the range from 0.5,1.0 and 1.6 to 80 mg/L with the LOD=0.2,0.3 and 0.6 mg/L,respectively. The mechanism of the host-guest action between β-CD and m, p, and o-nitrophenol was also discussed. This method was applicable to the analysis of industry waste wate including nitrophenol.

关 键 词：毛细管电泳 方波安培检测 硝基苯酚 位置异构体 Β-CD 

分 类 号：O657.8[理学—分析化学]