荧光定量PCR检测HBV-DNA与乙肝两对半检测结果的相关性探讨  

Correlation of Detection of HBV-DNA by Fluorescent Quantitative PCR with Routine Detection of HBV

作　　者：张蓓[1] 杨晓云[1] 刘蕊[1] 纪凤祥[1] 

机构地区：[1]天津市人民医院检验科,天津300121

出　　处：《河北医学》2006年第9期835-838,共4页Hebei Medicine

摘　　要：目的:探讨HBV-DNA的检出情况与乙肝两对半结果之间的关系。方法:运用荧光定量PCR(FQ-PCR)检测307例疑似乙肝患者血清中的HBV-DNA,同时运用ELISA方法进行两对半指标的检测,并对结果进行相关性探讨。结果:HBsAg(+)HBeAg(+)HBcAb(+)组(大三阳组)患者血清HBV-DNA检出率为98.15%(53/54),平均拷贝数为7.56E+06/m l;HBsAg(+)HBeAb(+)HBcAb(+)组(小三阳组)患者血清HBV-DNA检出率为27.78%(15/54),平均拷贝数为3.79E+05/m l;HBsAg(+)HBcAb(+)组血清HBV-DNA检出率为91.18%(31/34),平均拷贝数为2.85E+05/m l,小三阳组HBV-DNA阳性检出率及拷贝数均低于大三阳组,且与大三阳组比较均具有显著性差异(P<0.01,P<0.05);HBsAb(+)组血清HBV-DNA检出率为1.6%(1/63),平均拷贝数为1.13E+08/m l;HBcAb(+)组血清HBV-DNA检出率为16.67%(1/6),平均拷贝数为3.00E+04/m l;HBsAb(+)HBeAb(+)HBcAb(+)组血清HBV-DNA检出率为12.50%(4/32),平均拷贝数为3.00E+05/m l;HBsAg(+)组血清HBV-DNA检出率为0(0/2);HBV-M全阴性组HBV-DNA检出率为1.61%(1/62),平均拷贝数为2.75E+05/m l。结论:HBV-M阴性患者仍有HBV-DNA阳性者,因此在检测中应联合应用两种方法进行检测,提高检出率,避免漏诊,为临床HBV感染、复制及传染性的判断以及指导治疗提供更为可靠的判定依据。Objective ：To explore the relationship between detection of HBV -DNA and routine detection of HBV. Method：HBV- DNA was detected with fluorescent quantitative PCR （FQ -PCR ） and HBV was routinely determined with ELISA in serum samples from 307 suspected cases of hepatitis B. Meanwhile , the correlation of results between the two methods was analyzed. Result：The detecting rate of HBV - DNA was 98.15% （53/54） and the mean copy number was 7.56E ＋06/ml in patients of HBsAg（ ＋ ） ,HBeAg（ ＋ ） and HBcAb（ ＋ ） （ group A ）, 27.78% （ 15/54 ） and 3.79E ＋ 05/ml in patients of HBsAg（ ＋ ）, HBeAb（ ＋ ） and HBcAb（ ＋ ） （group B） , 91.18% （31/34）and 2.85E ＋05/ml in patients of HBsAg（ ＋ ） and HBcAb （ ＋ ） （ group C ）. The 2 parameters were significandy lower in group B than in group A （ P 〈 0.01 and 0. 05）. Furthermore,they were respectively 1.6% （1/63） and 1.13E ＋08/ml in patients of HBsAb（ ＋ ）, 16. 67% （ 1/6） and 3. OOE ＋ 04/ml in patients of HBcAb（ ＋ ）, 12.50% （4/32） and 3. OOE ＋ 05/ml in patients of HBsAb（ ＋ ）, HBeAb（ ＋ ） and HBcAb（ ＋ ） ,0（0/2） in patients of HBsAg（ ＋ ） and 1.61% （ 1/62）and 2.75E ＋05/ml in HBV - M - negative patients. Conelusion：HBV - DNA positivity may appear in HBV - M - negative patients. Therefore, the 2 methods should be simultaneously used in the detection of HBV infection to promote the detecting rate to provide more reliable evidence for judgment of HBV infection, duplication and infectivity and its treatment in clinical practice.

关 键 词：常规检查 HBV-DNA 荧光定量PCR 

分 类 号：R446.62[医药卫生—诊断学]