烟草叶和根细胞质膜Ca^(2+)-ATPase特性的比较研究  

Comparison of the Plasma Membrane Ca^(2+)-ATPase Characterization from Tobacco Leaf and Root

作　　者：庄国庆[1] 邹琴[1] 韩善华[1] 

机构地区：[1]四川师范大学细胞生物学研究室,成都610066

出　　处：《四川大学学报（自然科学版）》2006年第4期903-907,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：四川省教育厅重点资助项目

摘　　要：测定了烟草叶和根细胞质膜Ca2+-ATPase的活性并对它们的特性进行了比较.结果表明,二者在许多方面都不尽相同.前者的最适pH为7.0,后者则为6.0,而且在一个相对较宽的pH范围内有高的活性.叶细胞质膜Ca2+-ATPase的最适反应温度为45℃,[ATP]0.5为2.2mmol/L,根细胞质膜Ca2+-ATPase则分别为40℃和1.9mmol/L.叶和根的细胞质膜Ca2+-ATPase均受Ca2+激活.曙红B(EB)对烟草叶和根细胞质膜Ca2+-ATPase有明显的抑制作用,当EB浓度达到50μmol/L后,抑制作用才不再有显著的增加.本文讨论了烟草叶和根细胞质膜Ca2+-ATPase特性出现差异的原因及其与环境的关系.Activities of the plasma membrance Ca^2 ＋ -ATPase from tobacco leaf and root were determined and their Characterizations were also compare. The results indicated they were different in many aspects. The activity of leaf plasma membrane Ca^2＋-ATPase reached the maximum at pH7.0 ,the root plasma membrane Ca^2＋-ATPase reached that at pH 6.0 and showed a high activity in a broader range of pH. The optimum reaction temprature of the leaf plasma membrane Ca^2＋-ATPase was 45℃ and that of the root plasma membrane Ca^2＋-ATPase was 40℃. The leaf and the root plasma membrane Ca^2＋-ATPase were activated by Ca^2＋, Their [ATP ]0.5 were 2.2 mmol/L and 1.9 mmol/L respectively. EB （Erythrosin B） could strongly inhibit the activities of the leaf and root plasma membrane Ca^2＋-ATPase, only after EB concentration reached 50 μmol/L, the increase of EB inhabition was not remarkable. The difference of the tobacco leaf and root plasma membrane Ca^2 ＋ -ATPase characterization and their relation with environment were discussed in this paper.

关 键 词：烟草 根 叶 细胞质膜 CA^2+-ATPASE 

分 类 号：Q735[生物学—分子生物学]