甘蓝型油菜Toc33基因启动子的克隆及序列分析  

Cloning and Sequence Analysis of Toc33 5' Regulation Region in Brassica napus

作　　者：李熠毅[1] 刘薇[1] 李雪东 王茂林[1] 赵云[1] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]成都生物制品研究所,成都610023

出　　处：《四川大学学报（自然科学版）》2006年第4期944-947,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金项目(30170500和30571174)

摘　　要：A 1400bp DNA fragment in 5’ region of Toc33 Brassica napus was cloned by an improved single primer PCR method.The result of DNA sequence analysis showed that the fragment consisted of two regions.One of 491bp was partial coding sequence of Toc33 gene,the other of 909bp was the promoter of Toc33 gene.Besides TATA-box and CAAT-box,the promoter sequence included several cis-acting elements which had relation to light-regulation of plant.The cis-acting elements were G-box,GATA-box,I-box,SORLIP1AT motif,CIACADIANLELHC motif and so on.As a result,it was presumed that the transcription activity of promoter of Toc33 gene from Brassica napus may be regulated by light.A 1400bp DNA fragment in 5＇. region of Toc33 Brassica napus was cloned by an improved single primer PCR method. The result of DNA sequence analysis showed that the fragment consisted of two regions. One of 491bp was partial coding sequence of Toc33 gene, the other of 909bp was the promoter of Toc33 gene. Besides TATA-box and CAAT-box, the promoter sequence included several cis-acting elements which had relation to light-regulation of plant. The cis-acting dements were G-box, GATA-box, I-box, SORLIP1AT motif, CIACADIANLELHC motif and so on. As a result, it was presumed that the transcription activity of promoter of Toc33 gene from Brassica napus may be regulated by light.

关 键 词：基因启动子 甘蓝型油菜 序列分析 克隆 基因编码区 蛋白转运 cDNA 叶绿体 PCR方法 植物细胞 

分 类 号：Q754[生物学—分子生物学] S565.4[农业科学—作物学]