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作 者:周蓉[1] 张小娟[1] 王贤智[1] 沙爱华[1] 周新安[1] 涂赣英[1]
机构地区:[1]中国农业科学院油料作物研究所农业部油料作物遗传改良重点开放实验室,武汉430062
出 处:《大豆科学》2006年第3期212-217,共6页Soybean Science
基 金:国家"863"重大专项(2002AA207007);农业部油料作物遗传改良重点开放实验室资助项目
摘 要:利用SSR标记和UPGMA聚类分析,对64份湖北省大豆种质资源进行遗传多样性分析。结果表明,27对SSR引物扩增出166条多态性带,SSR的遗传多样性指数分布范围,Simpson指数为0.2406-0.9037,平均值0.6960;Shannon—weaver指数为0.4800-2.4684,平均值1.4538。聚类分析将湖北省地方大豆品种分为3个类群,包括鄂中南江汉平原大豆类群,鄂西北大豆类群以及鄂东、鄂南和鄂西混合类群。江汉平原大豆地方品种表现出较高的遗传多样性水平。There were more than one thousand local soybean [- Glycine max (L.) Merr. ] germplasms in Hubei province. However, their diversity was not well characterized. The objective of this study was to quantify genetic diversity of sixty-four soybean landraces from Hubei by SSR and cluster analysis with the UPGMA method. 166 polymorphic bands were amplified by twenty-seven pairs SSR primers. Genetic diversity index showed that distribution of Simpson index was 0. 2406-0. 9037 and mean 0. 6960, and distri- bution of Shannon-weaver index was 0. 4800-2. 4684 and mean 1. 4538. By using the dendrogram analysis, 64 landraces were separated into three major groups at the DNA level: Jianghan alluvial plain group, northwest Hubei group and mixed group of the east, south and west of Hubei province. The diversity level of soybean landrace from Jianghan alluvial plain was higher than that of other landraces.
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