大豆SMV3号株系抗病基因的SSR标记  

IDENTIFICATION OF SSR MARKER LINKED TO THE RESISTANCE GENE OF SMV3 IN SOYBEAN

作　　者：滕卫丽[1] 李文滨[1] 邱丽娟[2] 韩英鹏[1] 赵桂云[1] 关荣霞[2] 常汝镇[2] 

机构地区：[1]东北农业大学大豆研究所国家教育部大豆生物学重点实验室,哈尔滨150030 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《大豆科学》2006年第3期244-249,共6页Soybean Science

基　　金：863项目(编号:2003AA207060)的部分研究内容

摘　　要：运用简单序列重复技术(SSR技术),采用改良的分离群体组群分析法(BSA法),对大豆品系中选95-5117(R)×HB1(S)的F5代重组自交系群体接种SMV 3号株系鉴定抗性,并进行抗病基因的分子定位。结果表明:中选95-5117对SMV 3号株系的抗性受一对基因控制。用Mapmaker/Exp3.0b进行连锁分析,该基因位于大豆染色体组的F连锁群上,并获得了与SMV3号株系抗病基因连锁的2个SSR标记Satt114和Satt362,遗传距离分别为2.3cM和8.6cM。标记与抗病基因间的排列顺序和距离为:Satt114-2.3 cM-RSMV3-8.6 cM-Satt362。Mapping for soybean mosaic virus （SMV） resistant gene in Fz,s population derived from Zhongx uan 95--5117 × HB1 was carried out with simple sequence repeat （SSR） markers by modified bulked segregation analysis （BSA）. Zhongxuan 95--5117 is resistant while HB1 is susceptible to SMV3 strain. SMV3 strain was inoculated on the parents and F2,5 population. The results showed that the resistance of Zhongxuan 95--5117 to SMV3 strain was controlled by one pair of genes. Mapmaker/Exp3.0b was used to study the linkage between markers and the resistant gene. Linkage analysis indicated that the resistance gene was mapped on the linkage group F and was linked to two SSR markers Sattll4 and Satt 362 with distances of 2.3 cM and 8.6 cM, respectively. The orientation of the gene and markers was determined as Satt114--2.3 cM--RSMV3--8.6 cM--Satt 362.

关 键 词：大豆 大豆花叶病毒 抗病基因 SSR标记 

分 类 号：S565.1[农业科学—作物学]