甘油脱水酶和二醇脱水酶β亚基生物信息学分析  

Bioinformatic Analysis of Genes Encoding β Subunits of Glycerol Dehydratase and Diol Dehydratase

作　　者：洪燕[1] 李雯君[1] 张燎原[1] 夏启容[1] 方柏山[1] 

机构地区：[1]工业生物技术福建省高等学校重点实验室(华侨大学),福建泉州362021

出　　处：《生物技术》2006年第4期1-4,共4页Biotechnology

基　　金：国家自然科学基金资助项目(20276026;20446004);福建省科技计划项目重点项目(2003I020)资助

摘　　要：依赖辅酶B12的甘油脱水酶和二醇脱水酶是同工酶。它们不仅氨基酸序列和蛋白质折叠方式非常相似,而且有着相同的催化机制。该文在同一菌株中将编码甘油脱水酶β亚基基因和编码二醇脱水酶β亚基基因片段分别克隆至T载体,从而获得两段核苷酸片段序列,将pddB序列提交Genbank数据库,获登陆号为DQ483052。同时结合PDB数据库中甘油脱水酶和二醇脱水酶的晶体结构,该文用生物信息学方法对所测序列进行了初步分析,并对两种同工酶的β亚基对脱水酶与辅酶的亲和力进行了比较。Glycerol dchydratase （GDHt） and diol dchydratase （DDH） are highly homologous isofunctional enzymes.It has been shown that these enzymes are similar in molecular masses, spatial folding and catalytical mechanism.In this work, genes encoding β subunit of coenzyme B12 - dependent GDHt and DDH have been cloned into T plasmid respectively The pddB sequence has been submitted to Genbank database and a accession number： DQ483052 has been given. With the crystal strutures of GDHt and DDH in PDB database, the sequences in this work have been an-alyzed by biolniormatic methods.The affinity of GDHt and DDH for coenzyme Bl2during catalytic process have been studied in this work also.

关 键 词：甘油脱水酶 二醇脱水酶 催化机制 辅酶B12 

分 类 号：Q557.3[生物学—生物化学] Q523.8