一株产苝醌类光敏剂丝状真菌AL18的液体发酵工艺研究  

Study on Liquid-state Fermentation Technique of A Fungus AL18 Producing Perylenequinones Derivation Photosentisizer

作　　者：卢明锋[1] 张月杰[1] 

机构地区：[1]山东理工大学生命科学学院,山东淄博255049

出　　处：《生物技术》2006年第4期62-65,共4页Biotechnology

基　　金：国家重点基础研究计划项目(973项目)("绿色化学农药先导结构及作用靶标的发现与研究";2003CB114400)

摘　　要：为研究一株丝状真菌AL18产艹北醌类光敏剂的液体发酵工艺。以马铃薯综合培养基为发酵培养基,采用单次单因素实验法,研究了液体摇瓶培养条件对艹北醌类光敏剂产量的影响。实验结果表明:液体摇瓶最适培养条件为250ml三角瓶装液量40ml,接种量7.5%,接种种龄40h,初始pH5.75,摇床转数180r/min,30℃振荡培养48h。在此培养条件下,采用单因素法筛选了发酵培养基中的碳源、氮源和无机离子,选用L9(34)对筛选到的绵白糖(A)、蛋白胨(B)、蚕蛹粉(C)、CuSO4.5H2O(D)进行了正交试验。经优化后的发酵培养基配方为:马铃薯200g/L,绵白糖30g/L,蛋白胨3g/L,蚕蛹粉12.5g/L,磷酸二氢钾1g/L,硫酸镁0.5g/L,硫酸铜0.05g/L,VB1100mg/L。对此发酵培养基配方进行了5次验证实验,艹北醌类光敏剂的平均产量为1.21g/L。Perylenequinones derivation（PQD ）is a novel photosentizer. The liquid fermentation technique of a fungi AL18 producing POD was studied in the paper. The best fermentation condition was determined by monosyllabic test. The result is： 40ml culture medium per 250ml nask, inoculating amount 7.5% ,rotating speed 180r/min, initial pH 5.75,30℃ fermenting 48h. Under the condition, carbon sourse and nitrogen sourse and mineral salt was sieved by monosyllabic test. The optimum culture medium was determined by orthogenal test L9 （3^4）. The optimized medium is： 200g/L photato extract, 30g/L white sugar, peptone 3g/L, silkworm pupa powder 12.5g/L, KH2 PO4 lg/L, MgSO4 0.5g/L, CuSO4 0.05g/L, Vitamin B1 100mg/L. Under this culture medium the PQD yield reached 1.21g/L.

关 键 词：光敏剂 苝醌衍生物 液体发酵工艺 

分 类 号：TQ460[化学工程—制药化工]