苏云金素发酵培养基的优化设计  

Optimized Fermentation Medium for the Production of Thuringiensin

作　　者：常亚飞[1] 陈守文[1] 喻子牛[1] 

机构地区：[1]华中农业大学生命科学技术学院/农业微生物学国家重点实验室/微生物农药国家工程研究中心,武汉430070

出　　处：《中国生物防治》2006年第3期190-194,共5页Chinese Journal of Biological Control

摘　　要：采用Plackett-Burman设计对出发培养基各种成分影响苏云金芽孢杆菌中华亚种CT43发酵合成苏云金素的效应进行评价,筛选出有显著影响效应的因素为葡萄糖和黄豆饼粉。利用最陡爬坡路径试验逼近最大苏云金素产出区域,找到后续试验中心点。综合中心组合设计和响应面分析法确定显著因素的最佳浓度。优化后培养基组成为(g/L):葡萄糖22.8g、黄豆饼粉32.5g、酵母抽提物3.0g、蛋白胨4.5g、玉米浆3.0g、KH2PO42.0g、MgSO4.7H2O 0.15g、MnSO4.H2O0.02g,苏云金素产量可达3.2g/L,比出发培养基提高2倍以上。Experiments was designed to optimize the fermentation medium of thuringiensin produced by Bacillus thuringiensis subsp, chinensis CT43. Initially, Plackett-Burman design was applied to evaluate the effectiveness of the related influential factors; the selected ones that affected the fermentation were glucose and soybean powder. Then the steepest ascent experiment was adopted to approach the optimal region of the medium composition. At last, the optimum composition of the fermentation medium was obtained by central composite design and response surface methodology, that was ： 22.8 g·L^-1of glucose, 32.5 g·L^-1 of soybean powder, 3.0 g· L^-1 of yeast extract, 4.5 g·L^-1 of peptone, 3.0 g· L^-1 of corn mash, 2.0 g·L^-1 of KH2PO4, 0.15 g＇L^-1 of MgSO4·7H2O and 0.02 g·L^-1 of MnSO4·H2O. The final yield of thuringiensin was 3.2 g· L^-1. After optimization, the yield of thuringiensin production was increased over twice compared with that in the original medium.

关 键 词：苏云金素 试验设计 发酵培养基 优化 

分 类 号：S476.11[农业科学—农业昆虫与害虫防治]