多粘类芽孢杆菌HY96-2对番茄青枯病的防治作用  

Effect of Paenibacillus polymyxa HY96-2 on Bacterial Wilt of Tomato

作　　者：徐玲[1] 王伟[1] 魏鸿刚[1] 沈国敏[1] 李元广[1] 

机构地区：[1]华东理工大学海洋生化工程研究所/生物反应器工程国家重点实验室,上海200237

出　　处：《中国生物防治》2006年第3期216-220,共5页Chinese Journal of Biological Control

基　　金：国家863项目(2002AA245011)

摘　　要：室内测定了拮抗菌株HY96-2对番茄青枯病病原菌Nb-1的毒力。平板培养法和双层培养基法结果表明,HY96-2对Nb-1具有较好的拮抗作用,其抑制率分别为20.1%和24.7%。通过温室盆栽,确定了HY96-2发酵液及其制成的生防制剂KDLD对番茄青枯病的最佳防治方法为浸根法。番茄苗浸1×105cfu/ml的HY96-2发酵液0.5h,栽入灭菌土后浇入该HY96-2发酵液100ml,处理后30d防效仍高达69.7%。温室防治试验结果表明,采用浸根法HY96-2发酵液施用后21d对番茄青枯病防效可达77.8%。生防制剂KDLD稀释100倍施用后20d防效可达81.5%。大田防治试验结果表明,生防制剂KDLD稀释100倍后使用,在番茄整个生育期对番茄青枯病都具有很好的防效,在收获末期的防效仍可达81.8%。The inhibition of biocontrol agent Paenibacillus polymyxa HY96-2 on Ralstonia solanacearum Nb-1 was determined in vitro. Result showed that the inhibition rates were 20.1% and 24.7% by dual culture and double layer culture methods, respectively. Greenhouse trials demonstrated that soaking root was the best way to use the ferment fluid of HY96-2 and its product KDLD to control tomato bacterial wilt disease. Control efficiencies were 77.8% at 21 days after soaking root with HY96-2 ferment fluid and 81.5% at 20 days after soaking with 100 times diluted KDLD. Field tests showed that 100 times diluted KDLD prevented the disease in all growing season of tomato with control efficiency 81.8% at harvest.

关 键 词：番茄 青枯病 多粘类芽孢杆菌 生物防治 

分 类 号：S482.292[农业科学—农药学] S436.412.15[农业科学—植物保护]