槲皮素对长波紫外线引起表皮癌细胞DNA损伤的预防作用  

Preventive effect of querection on ultraviolet A-induced DNA damage in human epidermoid carcinoma cells

作　　者：周敏[1] 陈洁[2] 

机构地区：[1]太原市中心医院皮肤科,太原030009 [2]第二军医大学长海医院血液科

出　　处：《山西医科大学学报》2006年第6期597-599,共3页Journal of Shanxi Medical University

摘　　要：目的观察长波紫外线引起人表皮癌细胞DNA链断裂损伤的情况,以及槲皮素对这种损伤的预防作用。方法用长波紫外线照射细胞,用单细胞凝胶电泳法检测DNA损伤。结果长波紫外线剂量依赖性地诱发表皮癌细胞的DNA链断裂损伤,损伤的高峰出现在照射1 h。表现为慧星细胞百分比的降低和彗星尾巴长度的缩短,并有量效关系。结论槲皮素可以预防长波紫外线照射引起的皮肤癌细胞DNA链断裂损伤。Objective To investigate the preventive effect of quercetin on DNA damage in human epidermoid carcinoma cells induced by ultraviolet A （UVA）. Methods Cells were treated with UVA radiation, and DNA strand breaks were studied through the comet assay. Results UVA radiation led to a dose-dependently increase of DNA strand breakage, and the peak damage occurred at 1 h UVA treatment. The results showed the decrease of comet cells and the weakness of comet tails. Conclusion Quercetin can prevent the DNA strand breaks in human epidermoid carcinoma cells induced by UVA.

关 键 词：槲皮素 紫外线 DNA损伤 慧星试验 

分 类 号：R969[医药卫生—药理学]