菠菜甜菜碱醛脱氢酶基因在烟草中的表达  

Expression of Gene of Betaine Aldehyde Dehydrogenase in Tobacco

作　　者：陆云华[1] 马立新[1] 张新[2] 

机构地区：[1]湖北大学生命科学学院分子微生物与基因工程实验室,湖北武汉430062 [2]宜春学院化学与生物工程学院,江西宜春336000

出　　处：《江西农业学报》2006年第4期62-65,共4页Acta Agriculturae Jiangxi

基　　金：国家"863"项目(2002AA227011);湖北省自然科学基金(2003ABA118)资助

摘　　要：构建了含菠菜甜菜碱醛脱氢酶基因BADH基因的重组植物表达载体pLM46,转化根癌农杆菌(Agrobacterium tumefaciens,LBA4404),用农杆菌介导的叶圆盘法对烟草(Nicotiana tabacumL.)叶片进行遗传转化。在含有潮霉素的MS筛选分化培养基上筛选,获基因转化烟草植株4株。经W estern blot等方法检测,证明BADH基因在烟草转化植株中得到了表达。The expressive carrier of pLM46 with BADH gene from aldehyde dehydrogenase containing lycie was constructed. And the pLM46 carrier was transformed into Agrobacteriun turnefaciens （LBA4404）, and then the Nicotiana tabacum is transformed through leaf disc mediated by Agrobacterium turnefaciens. Having been selected on the screening medium containing hygromycin, 4 transformants appeared. Through testing by Western blot, the BADH gene in transformants has been expressive.

关 键 词：BADH基因 遗传转化 表达 烟草 

分 类 号：Q78[生物学—分子生物学]