Bioeffects of different functionalized silica nano-particles on HaCaT cell line  

作　　者：HE Xiaoxiao LIU Fang WANG Kemin GE Jia QIN Dilan GONG Ping TAN Weihong 

机构地区：[1]State Key Laboratory of Chemo/Biosensing and Chemometrics, Engineering Center for Biomedicine, Institute of Life Science and Biological Technology, Engineering Research Center for Bio-Nanotechnology of Hunan Province, Changsha 410082, China

出　　处：《Chinese Science Bulletin》2006年第16期1939-1946,共8页

基　　金：This work was supported by the Key Project of Natural Science Foundation of China （Grant No. 20135010）;National Key Basic Research Program （Grant No. 2002CB513100-10）;Key Technologies Research and Development Program （Grant No. 2003 BA310A16）;High-Tech Research and Development （863） Program （Grant No. 2003AA302250） ;the National Natural Science Foundation of China （Grant No. 20405005）.

摘　　要：The bioeffects of silica nanoparticles (SiNP), phosphorylate-terminated nanoparticles (PO4- NP) and amino-terminated nanoparticles (NH2NP) on HaCaT cell line have been studied in this paper. The effects of the three kinds of functionalized silica nanoparticles on adherence, proliferation and cycle of HaCaT cells have been investigated. And the cel- lular uptake of the three kinds of functionalized silica nanoparticles by HaCaT cells has also been exam- ined. Results indicated that the bioeffects of the three kinds of functionalized nanoparticles on HaCaT cells were concentration-dependent. And the three kinds of functionalized nanoparticles all exhibited well bio- compatibility if the concentration was below 0.2 μg/μL. While the cytotoxicities of the three kinds of function- alized nanoparticles on HaCaT cells would increase with the increasing of nanoparticles concentration, and the following order was observed: NH2NP > SiNP > PO4NP. In addition, the quantity and rapidity of cellular uptake of nanoparticles by HaCaT cells were diverse due to the different functional groups. Under the same conditions, NH2NP was most and fast internalized by HaCaT cells, followed by SiNP, and PO4NP was the least and slowest. These results provided theoretical foundation for the safe applica- tion and further modification of silica nanoparticles, which could broaden the application of silica nano- particles in biomedicine.The bioeffects of silica nanoparticles （SiNP）, phosphorylate-terminated nanoparticles （PO4- NP） and amino-terminated nanoparticles （NH2NP） on HaCaT cell line have been studied in this paper. The effects of the three kinds of functionalized silica nanoparticles on adherence, proliferation and cycle of HaCaT cells have been investigated. And the cellular uptake of the three kinds of functionalized silica nanoparticles by HaCaT cells has also been examined. Results indicated that the bioeffects of the three kinds of functionalized nanoparticles on HaCaT cells were concentration-dependent. And the three kinds of functionalized nanoparticles all exhibited well biocompatibility if the concentration was below 0.2 μg/μL. While the cytotoxicities of the three kinds of functionalized nanoparticles on HaCaT cells would increase with the increasing of nanoparticles concentration, and the following order was observed： NH2NP 〉 SiNP 〉 PO4NP. In addition, the quantity and rapidity of cellular uptake of nanoparticles by HaCaT cells were diverse due to the different functional groups. Under the same conditions, NH2NP was most and fast internalized by HaCaT cells, followed by SiNP, and PO4NP was the least and slowest. These results provided theoretical foundation for the safe application and further modification of silica nanoparticles, which could broaden the application of silica nanoparticles in biomedicine.

关 键 词：SiNP PO4NP 纳米粒子 HaCaT细胞系 生物效应 

分 类 号：Q813[生物学—生物工程]