Exogenous cAMP upregulates the expression of glnII and glnK-amtB genes in Sinorhizobium meliloti 1021  

作　　者：TIAN Zhexian MAO Xianjun SU Wei LI Jian BECKER Anke WANG Yiping 

机构地区：[1]National Laboratory of Protein Engineering and Plant Genetic Engi- neering, College of Life Sciences, Peking University, Beijing 100871, China [2]Lehrstuhl fuer Genetik, Universitaet Bielefeld, Postfach 100131, 33501 Bielefeld, Germany

出　　处：《Chinese Science Bulletin》2006年第16期1982-1985,共4页

基　　金：This work was supported by the Chinese National Key Program for Basic Research （973） （Grant Nos. 2001CB108902 ＆ 2001CB108903）;the National Natural Science Foundation of China （Grant No. 30470940）;Bundesministerium fiir Bildung und Forschung, Germany （Grant No. 031U213D）;Deutsche Forschungsgemeinschaft （Grant No. BIZ 7）.

摘　　要：The existence of multiple adenylate cy- clase encoding genes implies the importance of cAMP in Sinorhizobium meliloti 1021. In this study, as a pioneer step of understanding cAMP roles, mi- croarray analysis on S. meliloti was carried out for the function of exogenous cAMP. To our surprise, the result showed that the transcriptions of glnII and glnK genes were significantly upshifted in the presence of exogenous cAMP in S. meliloti. This phenomenon is further confirmed in S. meliloti that the expression of either glnII or glnK promoter-lacZ translational fusion is higher in the presence of exogenous cAMP. Therefore, for the first time, we have identified genes from S. meliloti whose expression is activated by cAMP. The potential physiological role of upregula- tion of glnII and glnK by cAMP is discussed.The existence of multiple adenylate cyclase encoding genes implies the importance of cAMP in Sinorhizobium meliloti 1021. In this study, as a pioneer step of understanding cAMP roles, microarray analysis on S. melilotiwas carried out for the function of exogenous cAMP. To our surprise, the result showed that the transcriptions of glnll and glnK genes were significantly upshifted in the presence of exogenous cAMP in S. meliloti. This phenomenon is further confirmed in S. meliloti that the expression of either glnll or glnK promoter-lacZ translational fusion is higher in the presence of exogenous cAMP. Therefore, for the first time, we have identified genes from S. meliloti whose expression is activated by cAMP. The potential physiological role of upregulation of glnll and glnKby cAMP is discussed.

关 键 词：CAMP 外生 基因表达 glnII glnK-amtB 

分 类 号：Q78[生物学—分子生物学]