流动注射化学发光法测定样品中的白藜芦醇  

Flow injection chemiluminescence determination of resveratrol based on its inhibitory effect on oxidation reaction between n-bromosuccinimide and luminol

作　　者：姚型军[1] 王术皓[1] 杜凌云[1] 

机构地区：[1]聊城大学化学化工学院,山东聊城252059

出　　处：《化学研究与应用》2006年第8期1005-1008,共4页Chemical Research and Application

基　　金：山东省中青年学术骨干和学科带头人培养基金资助项目

摘　　要：In alkaline medium,a new flow injection chemiluminescent method has been developed for the determination of resveratrol,based on the inhibition of the chemiluminescence reaction between luminol and N-bromosuccinimide by resveratrol.Conditions of this chemiluminescent reaction have been discussed and optimized.The linear range of determination is 6.0 10-7-1.0 10-5mg/mL for resveratrol.The detection limit is 8.15 10-8mg/mL.And the relative standard deviation（RSD）（n=11） is 0.35% for 5.0 10-6mg/mL resveratrol.The method has been applied to determine the content of resveratrol in grape skin,grape wine and traditional Chinese medicine polygonumcuspidatum seib with satisfactory results.The possible mechanism of the chemiluminescence reaction was also studied.In alkaline medium, a new flow injection chemilumineseent method has been developed for the determination of resveratrol, based on the inhibition of the chemiluminescence reaction between luminol and N - bromosuccinimide by resveratrol. Conditions of this chemiluminescent reaction have been discussed and optimized. The linear range of determination is 6.0 10^-7 - 1.0 10^-5mg/mL for resveratrol. The detection limit is 8. 15 10^-8mg/mL. And the relative standard deviation （RSD） （n = 11 ） is 0. 35% for 5.0 10^-6mg/mL resveratrol. The method has been applied to determine the content of resveratrol in grape skin, grape wine and traditional Chinese medicine polygonumcuspidatum seib with satisfactory results. The possible mechanism of the chemiluminescence reaction was also studied.

关 键 词：N-溴代丁二酰亚胺(NBS) 鲁米诺(Luminol) 化学发光 白藜芦醇(RES) 

分 类 号：O657.32[理学—分析化学]