草乌花煎煮过程中三酯型乌头生物碱的酯交换反应  

Ester-exchange reactions for triester aconitines in decocting flowers of Aconitum kusnezoffii

作　　者：王勇[1] 宋凤瑞[2] 刘志强[2] 刘淑莹[2] 

机构地区：[1]深圳大学生命科学学院深圳市微生物基因工程重点实验室,广东深圳518060 [2]中国科学院长春应用化学研究所新药研究室,吉林长春130022

出　　处：《中草药》2006年第8期1156-1158,共3页Chinese Traditional and Herbal Drugs

基　　金：深圳市科技计划资助项目(200528)

摘　　要：目的研究草乌花煎煮过程中脂类生物碱的变化。方法利用电喷雾离子阱质谱分析,比较草乌花乙醇提取液、草乌花单煎液和草乌花煎煮后残渣中的生物碱。结果在草乌花单煎液中易溶于水的别那宁质量分数显著提高,未检测到脂肪酸酯型生物碱,三酯型脂类生物碱成为煎煮过草乌花中的主要生物碱。结论在草乌花煎煮过程中,双酯型和三酯型乌头生物碱除发生水解反应外,还发生酯交换反应生成双酯型和三酯型脂类生物碱。三酯型乌头生物碱的C8位乙酰基被各种长链脂肪酰基取代的酯交换反应为首次报道。Objective To study how and why the lipo-alkaloids changed in decocting the flowers of Aconitum kusnezoffii （FAK）. Methods The alkaloids in ethanol extract of FAK, FAK decoction, and the residues of decocted FAK were analyzed and compared by electrospray ionization tandem mass spectrometry （ESI-MS^n）. Results No lipo-alkaloids were detected in FAK decoction, however, triesterlipo-alkaloids become the dominant components in the residues of FAK decoction. Conclusion Besides hydrolysis reactions, ester-exchange reactions happen for diester-aconitines and triester-aconitines in the decocting of FAK. It is the first time to report that Cs-acetyl is displaced by long chain fatty acyl for triester-aconitines in the ester-exchange reactions.

关 键 词：草乌花 酯交换反应 电喷雾串联质谱 

分 类 号：R286.02[医药卫生—中药学]