石菖蒲及其有效成分α-细辛醚对癫痫幼鼠脑海马神经元凋亡的影响  

Effect of Acorus gramineus and its active component α-asarone on apoptosis of hippocampal neurons in epileptic young rats

作　　者：杨立彬[1] 李树蕾[2] 黄艳智[3] 王宇虹[4] 梁建民[1] 张淑琴[4] 

机构地区：[1]吉林大学第一医院儿科,吉林长春130021 [2]吉林大学基础医学院组胚教研室,吉林长春130021 [3]长春市儿童医院,吉林长春130051 [4]哈尔滨医科大学附属第一临床医院神经科,黑龙江哈尔滨150001

出　　处：《中草药》2006年第8期1196-1199,共4页Chinese Traditional and Herbal Drugs

基　　金：吉林省科技厅资助项目(20030430);吉林省中医药管理局资助项目(2004079)

摘　　要：目的探讨石菖蒲及其有效成分α-细辛醚对戊四氮(PTZ)诱发的幼鼠癫痫发作所激发的海马区神经元凋亡的影响。方法利用光电镜技术观察癫痫幼鼠海马神经元的病理改变,TUNEL法检测凋亡细胞,免疫组织化学法检测Bax和Bcl-2的表达情况。结果致痫对照组癫痫幼鼠海马CA1和CA3区大量神经元凋亡;药物治疗后凋亡细胞数明显减少(P<0.01);PTZ致痫各组幼鼠海马CA1和CA3区Bcl-2和Bax阳性细胞数量较正常对照组均有明显增加(P<0.01)。其中,苯巴比妥钠组、石菖蒲组、α-细辛醚组Bcl-2阳性细胞数量增加较致痫对照组显著(P<0.01);Bax阳性细胞数量各致痫组之间无显著性差异。正常对照组Bcl-2/Bax的值约为6.0;致痫对照组约为0.7;其余3组均约为1.0。结论石菖蒲和α-细辛醚可能通过增强Bcl-2表达,抑制幼鼠癫痫发作激发的海马神经元凋亡。Objective To explore the effect of Acorus gramineus and its active component a-asarone on apoptosis of hippoeampal neurons in young rats with epilepsy induced by pentylenetetrazol （PTZ）. Methods The pathological changes of hippocampal neurons were observed by light microscope and electronic microscope. The apoptotic cells were detected by TUNEL and the expression of Bax and Bcl-2 were detected by immunohistoehemistry. Results There were numerous apoptosis of hippocampal neurons in CA1 and CA3 region in young rats of epilepsy control group. After medication, the number of apoptotic neurons was decreased significantly （P〈0. 01）. The number of Bcl-2 or Bax positive cells of CA1 and CA3 region in all of four group of epileptic young rats induced by PTZ was increased obviously compared with normal control group （P〈0.01）. The number of Bcl-2 positive cells in phenobarbital sodium, A. grarnineus, and α-asarone groups was more than that of epilepsy control group significantly （P〈0.01）. But the number of Bax positive cells was no markedly difference among the each groups. The ratio of Bcl-2 vs Bax was approximate 6.0 in epilepsy control group, 0. 7 in phenobarbital sodium group, 1.0 in other three groups. Conclusion A. grarnineus and α-asarone could prevent neurons from apoptosis induced by epileptic attack in young rats.

关 键 词：石菖蒲 α-细辛醚 癫痫 苯巴比妥钠 凋亡 

分 类 号：R285.5[医药卫生—中药学]