巨细胞病毒感染人脐静脉内皮细胞后RAGEmRNA的时序性表达及其意义  

Sequential changes of RAGE mRAN in human umbilical vein endothelial cells with human cytomegalovirus infection

作　　者：吴建敏[1] 周亚滨[1] 程轶喆[1] 李树英[1] 唐伟[1] 赵蔚明[1] 贾继辉[1] 栾怡[1] 齐眉[1] 王红[1] 刘娟[1] 于晗[1] 于修平[1] 

机构地区：[1]实验畸形学教育部重点实验室山东大学医学院微生物学教研室,山东济南250012

出　　处：《山东大学学报（医学版）》2006年第8期757-759,769,共4页Journal of Shandong University:Health Sciences

基　　金：山东大学跨学科研究课题(21310053182042);山东省卫生厅课题(413940)

摘　　要：目的:研究人巨细胞病毒(HCMV)感染对人脐静脉内皮细胞(HUVEC)糖基化终产物受体(RAGE)表达的影响,探讨HCMV感染致动脉粥样硬化的作用机制。方法:用HCMV感染HUVEC,采用RT-PCR方法检测不同感染时段细胞RAGEmRNA的表达。结果:HCMV感染HUVEC后,0 h时RAGEmR-NA有基础水平的表达,4 h开始升高,8 h时表达水平明显升高,随感染时间延长,表达量逐渐增高,感染24 h时达高峰,48 h开始回落,72 h表达量仍维持在较高的水平,显著高于0 h。各时段与0 h比较,均有统计学意义(P<0.01)。结论:HCMV感染人脐静脉内皮细胞后能够促进RAGE mRNA的表达,并且呈时间依赖性。HCMV有可能通过上调RAGE介导内皮细胞的炎症反应,促进动脉粥样硬化的发生、发展。Objective： To study the mechanims of cytomegalovirus infection by investigating the sequential changes of the receptor for advanced glycation end products in human umbilical vein endothelial cells with human cytomegalovirus infection. Methods： HUVEC was cultured and then infected by HCMV. Expression of RAGE mRNA was detected by reverse transcriptase polymerase chain reaction. Results： RAGE mRNA was expressed at a low level in control group, and began to increase 4 hours after HCMV infection, rose to a significant level at 12 hours, reached the peak at 24 hours. After 48 hours, it declined obviously and remained a relatively high level at 72 hours （ P 〈 0.01）. Conclusions： HCMV infection can enhance the expression of RAGE mRNA in HUVEC in a time-effect fashion, and may induce the inflammatory reaction by up-regulating RAGE expression on endothelial cells, which facilitate the occurrence and development of atherosclerosis.

关 键 词：人巨细胞病毒 感染 受体 糖基化终产物 人脐静脉内皮细胞 

分 类 号：R373[医药卫生—病原生物学]