糖尿病大鼠肾脏RAGE的表达与足细胞损伤的相关性研究  

Exression of RAGE in diabetic rat kidney tissue: relation between RAGE and podocyte injury

作　　者：张丽萍[1] 蒋玲[1] 侯志强[1] 

机构地区：[1]山东大学齐鲁医院内分泌科,山东济南250012

出　　处：《山东大学学报（医学版）》2006年第8期802-805,818,共5页Journal of Shandong University:Health Sciences

摘　　要：目的:探讨大鼠肾组织中糖基化终产物受体的表达和肾小球足细胞的相关性及其在糖尿病肾病中的临床意义。方法:用免疫组化方法检测链脲佐菌素(STZ)诱发糖尿病及正常对照Wistar大鼠肾脏中糖基化终产物受体、结蛋白的表达,采用图像分析软件定量计数肾小球足细胞数。对大鼠肾小球糖基化终产物受体的表达与肾小球足细胞进行相关分析。结果:与对照组相比,糖尿病组大鼠肾小球糖基化终产物受体、结蛋白表达明显增加(P<0.01),肾小球足细胞明显减少(P<0.01);大鼠肾小球糖基化终产物受体的表达与肾小球足细胞呈负相关(r=-0.736,P<0.01)。结论:RAGE介导的细胞信号途径可能参与了糖尿病时足细胞病变的发生。Objective： To evaluate the changes of RAGE（receptor for advanced glycation endproducts） expression and podocytes in renal tissues of diabetic and control rats, and explore the relation between RAGE and podocyte injuries. Methods： Adult male Wistar rats were injected intravenously with streptozotocin （STZ） to induce diabetes. The rats were sacrificed eight weeks after the induction of diabetes. The renal RAGE and desmin expressions were accessed by semi-quantitative immunohistochemistry. The average podocyte number of each glumerulus was determined by dividing the total podocytes by the glomerular profile area as determined by point counting. And correlation analysis was used to evaluate the relationship between RAGE expression and podocytes of each glomerulus. Resuits： Compare with control rats, the expressions of RAGE and desmin were increased remarkably（ P 〈 0.01 ）, and the podocytes of each glomemlus was decreased significantly（P 〈 0.01） in diabetic mrs. There was a negtive relationship between the RAGE expression and the podocytes of each glomcrulus（ r = - 0. 736, P 〈 0.01 ）. Conclusion： RAGE may play an important role in the progression of diabetic ncphropathy.

关 键 词：糖尿病肾病 受体 糖基化终产物 肾小球足细胞 结蛋白 大鼠 WISTAR 

分 类 号：R587.2[医药卫生—内分泌] R-332[医药卫生—内科学]