紫杉醇纳米粒注射液在荷瘤小鼠体内的组织分布及抗肿瘤作用  

The tissue distribution and antitumor effect of nano-paclitaxel injection in tumor-bearing mice

作　　者：高彦慧[1] 厉保秋[2] 甘一如[3] 董欣 程秀民[1] 赵燕彪 

机构地区：[1]山东大学药学院,山东济南250012 [2]山东大学毒理学研究所,山东济南250012 [3]天津大学生命科学与工程研究院,天津300072 [4]山东弘立医学动物实验研究有限公司,山东济南250025

出　　处：《山东大学学报（医学版）》2006年第8期823-826,830,共5页Journal of Shandong University:Health Sciences

基　　金：国家863计划资助课题(No.2002AA2Z3138)

摘　　要：目的:研究抗癌药物紫杉醇纳米粒注射液在荷瘤小鼠体内的组织分布及抗H22肝癌的作用。方法:将昆明种小鼠前肢腋皮下接种H22肝癌细胞,待瘤体长到1 g,分别尾静脉注射紫杉醇纳米粒注射液及市售紫杉醇注射液,采用液相色谱-质谱-质谱联用的方法测定荷瘤小鼠体内不同组织中紫杉醇的含量。结果:两种不同制剂的紫杉醇主要分布于胰腺、卵巢、脾脏,其抗肿瘤作用以2.5 mg/kg的紫杉醇纳米粒注射液更有效;紫杉醇纳米粒注射液在胰腺、卵巢、脾脏、实体瘤中的分布明显高于紫杉醇注射液(P<0.05);其它组织器官分布差异无统计学意义(P>0.05)。结论:两种紫杉醇注射液均有明显抗肿瘤作用,但紫杉醇纳米粒注射液在胰腺、卵巢、实体瘤的靶向性高于紫杉醇注射液,其分布与疗效有一定关联性。Objective： To study the tissue distribution and antitumor effect of a new preparation paclitaxel-loaded nanoparticles （PCT-N） injection in tumor-bearing mice. Methods： Kunming breed mice were subcutaneously inoculated hepatoma H22 cell to the armpit of forelimb, and then injected PCT-N and Toxal injection （PCT） into tail vein. The concentration of pachtaxel in tissue was determined by liquid chromatography-tandem mass spectrometry. Results： Paclitaxel was mainly found in pancreas, ovary and spleen and it was higher in PCT-N than in PCT （P〈0.05）, and PCT-N on the concentration of 2.5 mg/kg had the most efficacious inhibiting effect on tumor, whereas there was no obvious difference between PCT-N and PCT in other tissues of tumor-bearing mice （ P 〉 0.05）. Conclusions： Both PCT-N and PCT have obvious inhibiting effect on tumor, and the targeting efficiency of PCT-N in pancreas, ovary and tumor is better than that of PCT, which is relative with the distribution of paclitaxel.

关 键 词：紫杉醇 纳米粒 液相色谱-质谱-质谱 组织分布 

分 类 号：R979.1[医药卫生—药品] R917[医药卫生—药学]