微生物细胞ATP释放条件研究  

Study on the Releasing Conditions of Microbial Cell ATP

作　　者：张菊梅[1] 吴清平[1] 吴慧清[1] 郭伟鹏[1] 李程思[1] 

机构地区：[1]广东省微生物研究所广东省菌种保藏与应用重点实验室

出　　处：《微生物学通报》2006年第4期100-105,共6页Microbiology China

基　　金：广东省科技攻关项目(No.2003B30902)

摘　　要：在ATP生物发光法微生物细胞ATP释放剂的筛选研究中，发现采用环糊精等环状化合物可以解除表面活性剂类细胞ATP释放剂对发光反应系统的抑制作用，其中，7．5g／L环糊精CD（a）能中和1．5g／L的表面活性剂Ec和Es、Et，可以完全排除Ec、Es、Et对发光反应的抑制作用。通过比较各种细胞ATP释放剂释放ATP效果，筛选、组合出以表面活性剂Ec为代表的微生物细胞ATP释放剂；通过优化实验，发现用0．25～0．50g／L的Ec处理菌液1～2min时，细胞ATP的释放效果最好，从而建立了室温条件下简便、快速的微生物细胞ATP释放方法和ATP释放剂对发光反应系统抑制的消除方法。In the screen of ATP releasing reagent for microbial cell, it was found that 7.5g/L cyclodextrin （a） could neutralize 1.5 g/L surfactants Ec, Es and Et, and completely obviated the analytical interference from surfactants used as ATP releasing rcagents. By comparing the effect of a variety of ATP releasing reagents, surfactant Ec was screened and compounded as microbial ATP releasing reagent. The optimization experiment indicated that by using 0. 25 -0. 50g/L Ec to treat cell suspension for 1 -2min, the best releasing effect had been achieved. As a result, an easy and rapid releasing method for releasing of microbial cell ATP was established, and a way of removing the analytical interference completely from ATP releasing reagent was set up in this study.

关 键 词：微生物 生物发光 ATP释放剂 抑制作用 筛选 

分 类 号：Q936[生物学—微生物学]