鼠李糖脂快速定量分析方法及其影响因素研究  

Study on Rapid Methods for Quantitative Analysis Rhamnolipid and Its Influence Factors

作　　者：卢国满[1] 刘红玉[1] 曾光明[1] 黄国和[1] 张慧[1] 张利[1] 

机构地区：[1]湖南大学环境科学与工程学院,长沙410082

出　　处：《微生物学通报》2006年第4期106-111,共6页Microbiology China

基　　金：国家高技术研究发展计划(863)项目(No.2004AA649370);湖南省自然科学基金(No.05JJ30021)

摘　　要：为探寻简单、快速的由铜绿假单胞菌生产鼠李糖脂的定量分析方法,对比分析了蒽酮-硫酸法、L-半胱氨酸-硫酸法、苯酚-硫酸法及其影响因素。结果显示,蒽酮-硫酸法优于其它两种方法,并得出了其最佳测试条件。发酵液中剩余的葡萄糖、上清液对鼠李糖脂定量分析的影响可以忽略,菌体和中层杂质对鼠李糖脂的定量分析有一定程度的影响。因而,分析时要去除菌体。而中层杂质对定量分析的影响可以通过制备含中层杂质的鼠李糖溶液标准曲线而消除。In order to find an easy and rapid quantitative analytical method to detect rhamnolipid produced by Pseudomonas aeruginasa, three methods, H2SO4-anthrone analysis method, L-cysteine-H2SO4 method and phenol-H2SO4 method, were compared in the present paper, and the influence factors were also considered. The results showed that H2SO4-Anthrone analysis method was better than the others and its optimal reaction condition was obtained. The influence to the quantitative analysis of rhamnolipid from the residual glucose and the top clean liquid layer in the ferment solution could be ignored. But the influence from the bacterial body and the middle layer of the ferment solution reached a certain degree. Thus, the bacterial body should be removed before measuring. However, the influence from the middle layer of the ferment solution could be avoided by making a standard curve which was made by using a rhamnose mixed with the middle layer ferment solution.

关 键 词：鼠李糖脂 铜绿假单胞菌 蒽酮-硫酸法 定量分析 

分 类 号：Q936[生物学—微生物学]