菲律宾蛤仔血淋巴溶菌酶活性的研究  

Study on activity of lysozyme in hemolymph of Ruditapes phili-ppinarum

作　　者：王文琪[1] 徐申波[1] 姜令绪[1] 杨宁[1] 张玉娜[1] 

机构地区：[1]莱阳农学院,山东青岛266109

出　　处：《海洋科学》2006年第8期46-49,共4页Marine Sciences

基　　金：青岛市自然科学基金资助项目(05-1-JC-87);莱阳农学院博士基金资助项目(Y0222)

摘　　要：通过不同的反应条件对菲律宾蛤仔（Ruditapes philippinarum）血淋巴溶菌酶活力的影响实验，发现各反应条件与菲律宾蛤仔血淋巴溶菌活力密切相关：随着血淋巴添加量的增加，菲律宾蛤仔血淋巴溶菌活力逐渐升高，添加量在150～250μL之间时，两者呈现直线关系；溶菌活力随着温度和pH的升高呈峰值变化，分别在反应温度为50℃和pH为6．4时达到最大值，但统计分析显示pH在5．2～6．4之间时，溶菌活力差异不显著（P〉0．05）；溶菌活力随着反应时间的延长逐渐升高，当反应时间大于100min后趋于稳定。这为探讨菲律宾蛤仔免疫力的不同变化提供基础性的数据。This paper studied the effects of hemolymph volume, temperature, pH and time on the property of lysozyme in the hemolymph of Ruditapes philippinarum. The results indicated that there was a close relation between the four reaction conditions and bacteriolytic activity. As the volume is in of hemolymph increased, the bacteriolytic activity in the hemolymph increased gradually, while the volume and the bacteriolytic activity showed linearity when the volume is in between 150μL and 250μL. As the reaction temperature and pH increased, the graphs of bacteriolytic activity plotted against dates all showed curves. The graphs of bacteriolytic activity had well defined peaks when the temperature equaled 50℃ and the pH equaled 6.4. The data of bacteriolytic activity had no marked difference in the ranges from 5. 2 to 6. 4 pH. As the reaction time increased, bacteriolytie activity increased gradually between 10min and 100min, while baeteriolytic activity remained at a constant level between 100, 110 and 120 min and there was no distinct difference（P〉0.05）. That provided base data of different infections on immune activity of R. philippinarum.

关 键 词：菲律宾蛤仔(Ruditapes philippinarum) 溶菌活力 温度 PH 

分 类 号：Q55[生物学—生物化学]