ω-芋螺毒素SO3对大鼠慢性神经痛镇痛作用及对血清IL-6浓度影响  

The Analgesic Effect of Continuous Intrathecal Administration of Omega-conotoxin SO3 on a Model of Neuropathic Pain and the Down-regulation of Serum Concentration of IL-6 in Rats

作　　者：冯泽国[1] 王红[1] 周晓薇[2] 颜光涛[1] 刘风云[2] 张宏[1] 黄培堂[2] 

机构地区：[1]中国人民解放军总医院麻醉科,北京100853 [2]中国人民解放军军事医学科学院生物工程研究所,北京100071

出　　处：《解放军药学学报》2006年第4期261-264,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：国家"863"计划资助项目;No.2003AA624150

摘　　要：目的观察持续鞘内注射新型特异性N型电压依赖性钙通道阻滞剂—ω-芋螺毒素SO3对慢性压迫性损伤所致的神经痛大鼠的长期镇痛效果,及其对神经痛大鼠血清IL-6浓度的影响。方法建立大鼠CCI模型,比较观察连续鞘内注射ω-芋螺毒素SO3后大鼠热刺激爪退缩阈值和冷水中抬足次数变化以及血清IL-6水平的改变。结果CCI大鼠术后出现热刺激爪退缩阈值缩短(P<0.01)、冷水中抬足次数增加(P<0.01);血清中IL-6水平明显升高(P<0.01)。持续鞘内注射生理氯化钠溶液对这两种阈值变化以及血清中IL-6水平无明显影响;持续鞘内注射ω-芋螺毒素SO3(60ng/h)能明显提高CCI大鼠热刺激爪退缩阈值(P<0.01),减少冷水中抬足次数(P<0.01);并显著降低血清中IL-6水平(P<0.01)。结论长期持续鞘内注射ω-芋螺毒素SO3可有效地治疗CCI大鼠的冷、热痛觉过敏,并显著降低CCI大鼠血清IL-6浓度。Aim To evaluate the effect of model of neuropathic pain and the down-regula continuous intrathecal administration of omega-conotoxin SO3 on a tion of serum concentration of IL-6 in a rat model of chronic eonstrietion injury. Methods CCI rat models were established. The threshold of thermal and cold allodynia were observed and the serum concentration of IL-6 was measured after continuous i. t. omega-eonotoxin SO3. Results In the rats undergoing CCI surgery, withdrawal times to thermal stimulation were signifieandy exhibited （ P 〈 0.01 ）, and with drawal frequencies to cold stimuli were signifieandy increased （ P 〈 0.01 ）. The serum concentration of IL-6 was signifieandy increased in rats of C group（ P 〈0.01 ）. But there was no difference in cold and thermal stimulation threshold and serum concentration of IL-6 between C group and CN group. Continuous i. t. omega-eonotoxin SO3 60ng/h produced a significant increase of withdrawal times to thermal stimulation （ P 〈0.01 ） ,and a significant reduction of withdrawal frequencies to cold stimuli （ P 〈 0.01 ）. The serum concentration of IL-6 in CS group was significantly decreased after continuous i. t. omega-eonotoxin SO3 （ P 〈 0.01 ）. Conclusion Continuous i. t. omegaeonotoxin SO3 produced significant antinoeieeptive effects on both cold and thermal hyperalgesia while decreasing the serum concentration of IL-6.

关 键 词：坐骨神经慢性挤压伤(CCI) 持续鞘内注射 ω-芋螺毒素SO3 血清IL-6 

分 类 号：R965[医药卫生—药理学]