野菊花提取物抑制呼吸道合胞病毒作用的体外实验研究  

Anti-respiratory Syncytial Virus Effect of the Extraction of Chrysanthemum Indicum in Vitro

作　　者：张振亚[1] 方学平[1] 刁志花[1] 曾瑞红[1] 梅兴国[1] 

机构地区：[1]中国人民解放军军事医学科学院毒物药物研究所,北京100850

出　　处：《解放军药学学报》2006年第4期273-276,共4页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的评价野菊花提取物体外抑制呼吸道合胞病毒的效果方法采用MTT和空斑减数实验法,以病毒唑为阳性对照,测定野菊花提取物对呼吸道合胞病毒的抑制作用,并就野菊花提取物对呼吸道合胞病毒抑制的作用机制进行了初步研究结果药物的半数有效剂量(EC50)为(60.9±2.41)μg·ml-1,MTT法测定其半数中毒浓度(CC50)为(2.03±0.08)mg·ml-1,选择指数(SI)>33,有意义,在不同时间给药,对病毒的抑制实验中发现呼吸道合胞病毒感染HEp-2细胞后2、4、6、8h给野菊花提取物均有抑制作用(P<0.01),穿入和吸附抑制实验表明其对病毒穿入过程和吸附过程同样均有明显的抑制作用(P<0.01)。Aim To evaluate the effect of anti-respiratory syncytial virus of the extraction of Chrysanthemum indicum in vitro. Methods The cytotoxicity and antiviral activity of the extraction of Chrysanthemum indicum were tested by the plaque reduction method and 3-（4,5-dimethylthiazole-2-yl） 2,5-diphenyl tetrazolium bromide） MTT assay,with ribavirin treat as the positive control drug. Results The median effective concentration （50% effective concentration, EC50 ） and the median cytotoxicity concentration （50% cytotoxicity concentration, CC50 ）of the extraction of Chrysanthemum indicum were （ 60.9 ± 2.41 ） μg · ml^ -1 and （ 2.03 ± 0.08 ） mg · ml^-1, respectively. The selectivity index [ SI ] （ CC50 for HEp-2 ： EC50 ） of it for RSV was 34. SI 〉 4 is considered significant. Moreover, the extraction of Chrysanthemum indicum gave time-course experiments in inhibiting RSV. In this assay testing its effect of on virual growth, it showed obvious effect of inhibition on RSV when the herb was added at 2,4,6h and 8h after infection （ P 〈 0.01 ）. By an inhibition assay for virus absorption and penetration during the contact between the virus and cells, the extraction of Chrysanthemum indicum inhibited RSV absorption on the cells （ P 〈 0. 01 ）and penetration into the cell （ P 〈 0. 01 ）. The extraction of Chrysanthemum indicum also displayed direct inactivation on RSV. Conclusion The extraction of Chrysanthemum indicum showed potent inhibition to RSV in vitro.

关 键 词：野菊花 呼吸道合胞病毒 抗病毒作用 

分 类 号：R965[医药卫生—药理学]