用STS标记检测矮秆基因Rht-B1b和Rht-D1b在中国小麦中的分布  

Distribution of Dwarfing Genes Rht-B1b and Rht-D1b in Chinese Bread Wheats Detected by STS Marker

作　　者：杨松杰[1] 张晓科[1] 何中虎[1] 夏先春[1] 周阳[1] 

机构地区：[1]中国农业科学院作物科学研究所/国家小麦改良中心,北京100081

出　　处：《中国农业科学》2006年第8期1680-1688,共9页Scientia Agricultura Sinica

基　　金：国家"863"重大专项(2002AA207003);"948"重大国际合作项目

摘　　要：目的明确矮秆基因在中国小麦中的分布,有助于改良小麦株高和提高产量潜力。方法选用中国主要麦区品种(系)239份,用STS标记检测矮秆基因Rht-B1b(Rht1)和Rht-D1b(Rht2)的分布规律,验证其PCR标记在分子标记辅助育种中的可用性。结果(1)Rht-B1b和Rht-D1b特异性STS标记可以准确检测小麦品种的Rht-B1b和Rht-D1b矮秆基因。(2)Rht-B1b基因在全国的平均分布频率为24.3%,新疆冬春麦区高达62.5%,长江中下游冬麦区为42.3%,黄淮冬麦区、北部冬麦区和西北春麦区分别为28%、25.8%和25%,北部春麦区和西南冬麦区分别为9.1%和8.3%,东北春麦区供试材料未携带Rht-B1b基因。(3)Rht-D1b基因在全国的平均分布频率为46.9%,北部春麦区和黄淮冬麦区分别为72.7%和69%,西南冬麦区、西北春麦区和北部冬麦区分别为38.9%、37.5%和35.5%,长江中下游冬麦区和新疆冬春麦区分别为23.1%和12.5%,东北春麦区供试材料未携带Rht-D1b基因。结论分子检测结果和系谱分析表明,中国小麦品种(系)携带的Rht-B1b矮秆基因来自St2422/464和农林10,Rht-D1b矮秆基因来自农林10号、水源86、辉县红和蚰包麦。[Objective] Understanding the distribution of dwarfing genes in Chinese wheat will be crucial for yield improvement. [Method] A total of 239 Chinese wheat cultivars and advanced lines from major wheat regions were detected by STS markers to understand the distribution of the dwarfing genes Rht-B1b （Rhtl） and Rht-D1b （Rht2）. [Result] The PCR-based markers could be used to test the presence of Rht-B1b and Rht-D1b in wheat cultivars. The average frequency was 24.3% for Rht-B1b gene and 46.9% for Rht-D1b gene, respectively. Frequencies in Northem Winter Wheat Zone, Yellow ＆ Huai River Facultative Winter Wheat Region, Middle ＆ Low Yangtze Valley Winter Wheat Region, Southwestem Winter Wheat Region, Northeastem Spring Wheat Region, Northem Spring Wheat Region, Northwestem Spring Wheat Region and Xinjiang Winter-Spring Wheat Region were 25.8%, 28%, 42.3%, 8.3%, 0%, 9.1%, 25% and 62.5% for Rht-B1b gene, and 35.5%, 69%, 23.1%, 38.9%, 0%, 72.7%, 37.5% and 12.5% for Rht-lDb gene, respectively. [Conclusion] Molecular markers and pedigree information confirmed that Rht-B1b is from St2422/464 and Norin 10, Rht-D1b is from Norin 10, Suwon 86, Huixianhong, and Youbaomai.

关 键 词：普通小麦 矮秆基因 Rht-B1b Rht-D1b 分子标记 

分 类 号：S512.1[农业科学—作物学] S512.103.2