高效液相色谱检测器——高分辨飞行时间质谱仪的研制  

Development of TOF-MS Used as the Detector of HPLC

作　　者：贾韦韬[1] 徐国宾[1] 姚均[1] 何坚[2] 周振[3] 陆豪杰[1] 杨芃原[1] 

机构地区：[1]复旦大学化学系,上海200433 [2]厦门大学机电工程系,福建厦门361005 [3]中国科学院广州地球化学研究所,广东广州510640

出　　处：《质谱学报》2006年第3期129-134,共6页Journal of Chinese Mass Spectrometry Society

基　　金：科技部"十五"科学仪器攻关重大项目(2001BA210A01);973基金(01CB510202);863重大专项基金(02BAC11A11);上海市创新团队基金(03DZ14024);上海市重大基础研究(04DZ14005);国家自然科学基金(20405003);863人肝专项基金(2004BA711A18);973人肝新技术基金(2004CB520804)支持

摘　　要：本文研制的质谱仪是具有电喷雾离子源和射频四极杆接口的高分辨飞行时间质谱仪(ESI-QQQ-TOF-MS)。该仪器的特点如下:采用可三维调节、带有加热雾化气的电喷雾源液质联用接口;采用由三组四极杆组成、可有效调制离子束的离子光学系统;采用正负双脉冲推斥和垂直引入方式;采用经优化设计的二级有网反射器。该仪器分辨本领优于11 000(Full Width at Half Maximum,FWHM),质量测定精度优于10×10-6,最低检测限低于3 fmol/μL。可作为高效液相色谱优良的质谱检测器。Time-of-flight mass spectrometry （TOF-MS） is widely used in protein chemistry and proteomies research due to its high sensitivity; high resolution and high accuracy. Recently two Q-TOF-MS analytical systems are developed. Both systems are equipped with eleetrospray ionization source which could be directly hyphenated with HPLC as an outstanding detector. The ESI-triple quadrupole-orthogonal aeeeleration-refleeting-TOF mass spectrometer has some promising features, such as a ESI source with heatable nebulizer settied on a smart triaxiality moving stage, an excellent ion-optics system composed with triple-quadruple, double-pulse acceleration, and a double stage reflector with homemade grids. The Gramicidin-S sample concentration below to 3 fmol/μL can be identified by these instruments under the optimized conditions. The mass range of these instruments is from 50 u to 6 000 u with a general ESI interface to the HPLC. The optimal mass resolution exceeds 11 000 （Full Width at Half Maximum, FWHM） and the signal/noise ratio of reserpine （10 pg/μL） is greater than 100.

关 键 词：飞行时间质谱 电喷雾 高效液相色谱检测器 

分 类 号：O657.63[理学—分析化学] O644[理学—化学]