鸡冠花红褪色光度法测定羟自由基  

Determination of Hydroxyl Radical by Using Amaranth Fading Spetrophotometry

作　　者：张东[1] 文松林[1] 高俊杰[1] 余萍[1] 

机构地区：[1]沈阳理工大学环境与化学工程学院,辽宁沈阳110168

出　　处：《沈阳理工大学学报》2006年第3期12-14,38,共4页Journal of Shenyang Ligong University

摘　　要：研究了以食用色素-鸡冠花红为指示,氧化褪色光度法测定Fenton反应产生的羟自由基.在硫酸介质中,羟自由基能够氧化鸡冠花红褪色,在520nm波长处测定吸光度值的变化,可间接测定羟自由基的量.该法所用仪器简单,试剂廉价易得,操作简便,稳定性好,可作为一种筛选羟自由基清除剂的方法.A new spectrophotometric method for determination of hydroxyl radical · OH produced by Fenton reaction is proposed. The hydroxyl radical · OH can discolor the amaranth by oxidation reaction in H2SO4 medium. The change in absorbance, △A, can be measured at 520nm and the amount of · OH can be determined indirectly by spectrophotometer, establishing a new method of determination of hydroxyl radical. It can be used as a convenient and rapid way for selecting hydroxyl radical scavenger for its good stability.

关 键 词：鸡冠花红 褪色光度法 羟自由基 

分 类 号：O657.32[理学—分析化学]