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机构地区:[1]浙江中医学院附属第二医院(杭州市中医院),310007 [2]浙江省金华市中医院
出 处:《医学研究杂志》2006年第8期22-24,共3页Journal of Medical Research
基 金:浙江省自然科学基金资助项目(编号:M303903)
摘 要:目的观察在不同浓度或不同时间范围内尿酸盐诱导人血管内皮细胞(endothelialcellofvessels,ECV)间粘附分子(ICAM-1)表达的作用。方法体外培养人血管内皮细胞,分别用710μmol/L、1070μmol/L、1430μmol/L等不同浓度的尿酸盐刺激,用流式细胞术(FCM)、逆转录-聚合酶链式反应(RT-PCR)技术测定血管内皮细胞ICAM-1的蛋白表达和基因表达。结果(1)低浓度及中浓度尿酸盐刺激组细胞ICAM-1的蛋白表达和基因表达均较空白对照组显著提高;而高浓度尿酸盐刺激组与空白对照组相比无显著差异(P>0.05)。(2)内皮细胞在中浓度尿酸盐刺激下,ICAM-1的基因表达和蛋白表达于8h、16h、24h均较空白对照组显著升高,但其基因表达于48h已开始下降,与空白对照组相比无显著性差异,蛋白表达在48h仍持续升高,呈现出时间依赖关系。结论说明尿酸盐能直接作用于人的血管内皮细胞,诱导炎症分子的超表达,这也可能是其促进动脉粥样硬化斑块形成的重要机制之一。Objective To investigate the expression of intercellular adhesion molecule (ICAM - 1 ) in vascular endothelial cells induced by different concentration of urate solutions, which were formulated successfully,in different time. Methods Cultured the vascular endothelial cell line in vitro and stimulated them with urate of 710μmol/L,1070μmol/L, 1430μmol/L. The expression of ICAM -1 in vascular endothelial cells was detected by FCM and RT-PCR. Results 710μmol/L,1070μmol/L urate medium increased significantly the expression of ICAM - 1 in vascular endothelial cells in comparison with the control( P 〈 0.05 ) while 1430μmol/L urate did not affect significantly the expression of ICAM - 1 on level of protein and gene. The gene expression of ICAM - 1 in vascular endothelial cells induced by urate was increased significantly in 8h, 16h and 24h. But the protein expression of ICAM -1 increased continuously from 8h to 48h significantly than the control. Conclusions Urate induces the expression of ICAM - 1 , and therefore it may play an important role during the atherogenesis.
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