PCR试纸条检测乙肝病毒DNA新方法研究  

Research of Assaying HBV-DNA using PCR Teststrip

作　　者：徐岱[1] 

机构地区：[1]浙江省杭州市第六人民医院,310014

出　　处：《医学研究杂志》2006年第8期102-103,共2页Journal of Medical Research

摘　　要：目的通过和荧光定量PCR法的对比,试验一种乙肝病毒DNA检测的新方法(PCR试纸条)的特异性和敏感性,并评价其应用前景。方法对比检测4组不同HBV-DNA拷贝数的标本,分析PCR试纸条法检测HBV-DNA的敏感性和特异性。结果在18例>107/ml的标本中检出了强阳性结果17例,27例105～107标本检出了中等阳性结果23例,在27例103～105标本中检出弱阳性结果22例,经统计学检验均有显著差异。而28例<103标本检测结果均为阴性。结论研究结果显示该PCR杂交试纸条法检测HBV-DNA能够简便快速地获得HBV-DNA半定量结果,不需荧光定量PCR仪,检测成本低,易于在基层医院推广使用。Objective Through comparative resaerch with florescent quantitative PCR, a new method （PCR teststrip） assaying HBVDNA is evaluated in sensitivity and specificity and its widespread application prospect is alse assessed. Methods By comparative assaying of 4 group of specimen of different HBVDNA concentration, the sensitivity and specificity of PCR teststrip is analyzed. Results In 18 cases of 〉 10^7/mL specimens, 17 were detected highly positive by PCR teststrip method, in 27 cases of 10^5 - 10^7 specimens 23 were intermediately positive, in 27 cases of 10^3 - 10^5 specimens 22 were weakly positive, showing statistical significance. All 28 cases 〈 10^3 specimens were found negative. Conclusions The comparative research shows that PCR teststrip method is a quick, cheap, convient semi -quantitative HBVDNA assaying technique and widespread application may be prospected in rural hospitals.

关 键 词：PCR试纸条法 乙肝病毒DNA 

分 类 号：R512.62[医药卫生—内科学] R446.61[医药卫生—临床医学]