烧伤合并人工海水浸泡后对实验兔肾脏病理形态学的影响  

Influence of Burn Injury with Artificial Seawater Immersion on Kidney Pathological Changes in Experimental Rabbits

作　　者：王大鹏[1] 孙笑非[1] 徐华彪[1] 韩善桥[1] 虞积耀[1] 

机构地区：[1]海军总医院海战伤救治研究中心,北京100037

出　　处：《中国比较医学杂志》2006年第9期533-536,共4页Chinese Journal of Comparative Medicine

基　　金：全军"十五"指令性课题(01-L011)

摘　　要：目的探讨烧伤合并人工海水浸泡后对实验兔肾脏病理形态学的影响。方法实验动物致伤后随机分为对照组(n=4)、单纯烧伤组(n=10,致伤后直接观察)、海水浸泡组(n=10,致伤后立即放入人工配制的海水中浸泡3 h);采用光镜和电镜对肾组织进行病理形态学检查,以及免疫组织化学检查。结果光镜下肾小球毛细血管扩张充血或毛细血管内皮肿胀,管腔皱缩变窄。细胞增多,毛细血管受挤压。肾近曲小管细胞浊肿,部分可见透明变性及空泡变。部分肾小管上皮坏死。并可见部分远曲小管上皮消失,管腔内可见大量蛋白管形。电镜下毛细血管内皮细胞肿胀,系膜细胞肥大,线粒体肿胀。足突变形、融合。肾小管上皮细胞线粒体肿胀空泡化。部分可见胞质细胞器解离,胞质密度增大。肾间质血管充血。结论烧伤合并人工海水浸泡后可导致实验兔肾脏病理形态学改变。Objective To investigate the influence of burn injury with artificial seawater immersion on kidney pathological changes in experimental rabbits. Methods The healthy rabbits were randomly divided into control group （n = 4）, simple burn injury group （n = 10, immediately studied after burn injury） and seawater immersion group （n = 10, the rabbits were immersed in the seawater for 3 hours after burn injury.） Light and electron microscopes were employed to examine the pathological changes of kidney. Immunohistochemistry was also performed. Results By light microscopy, we found the dilation and hyperemia of glomerular capillaries, or swelling of endothelial cells. Glomerular cells increased and capillaries condensed. Hydrolic swelling in the epithelial cells of proximal convoluted tubules, hyaline degeneration and vacuolation were observed. The epithelial cells of some distal convoluted tubules disappeared and protein casts were found in the cavities. By electronmicroseopy, the swelling of endothelial cells, hypertrophy of mesangial cells and enlargement of mitochondria were found. Deformation and confluence of foot processes were also observed. Some mitochondria of the epithelial cells of renal tubules demonstrated vacuolation. Cytoplasm of some cells condensed. Hyperemia was found in the kidney mesenchyme. Conclusion The pathological changes of kidney appear to be associated with burn injury with artificial seawater immersion in experimental rabbits.

关 键 词：海水浸泡 烧伤 肾脏 病理形态学 

分 类 号：R826.54[医药卫生—临床医学]