实时定量PCR检测体外诱导条件下小鼠骨髓基质细胞S100mRNA表达水平变化  

Detection of S100 mRNA Expression in Induced-Bone Marrow Stromal Cells In Vitro by Real-Time PCR

作　　者：栾文杰[1] 王洁[1] 邵晨昕[1] 丁斐[1] 顾晓松[1] 

机构地区：[1]南通大学江苏省神经再生重点实验室,江苏226001

出　　处：《交通医学》2006年第4期373-375,F0002,共4页Medical Journal of Communications

基　　金：国家自然科学基金资助项目(30540063);江苏省自然科学基金项目(BK2005202)

摘　　要：目的:检测S100 mRNA含量,初步探讨骨髓基质细胞体外诱导条件下向雪旺细胞样细胞分化的可能性。方法:采用差速贴壁的方法分离、培养小鼠骨髓基质细胞。经β-ME,ATRA,Forskolin,bFGF,PDGF,HRG体外诱导后,利用实时定量PCR检测S100 mRNA水平的变化。结果:诱导后,骨髓基质细胞S100 mRNA水平上升,诱导前后水平变化有统计学意义(P<0.05)。结论:实时定量PCR检测S100 mRNA含量具有较高的灵敏度和特异性。体外诱导后骨髓基质细胞S100 mRNA表达量增多。objective： To establish real-time fluorescence quantitative reverse transcription-polymerase chain reaction（Real-time PCR）for quantification of S100 mRNA,and to explore the possibility of the differentiation of MSCs to Schwann-like cells in vitro. Methods： MSCs were isolated from hematopoietic stem cells by their strong adherence, and treated with beta-mecraptoethanol ,followed by retinoic acid and cultured in the presence of forskolin,basie-FGF,PDGF and HRG. Then real-time PCR was performed to detect S100 mRNA expression.Results：Induced MSCs enhanced expression of S100 mRNA, and there was difference in the S100 mRNA expression level between un-induced and induced MSCs（P〈0.05）.Conclusions：Real-time PCR assay has high sensitivity and specificity in detection of S100 mRNA expression level. Induced MSCs enhanced expression of Schwann ceils marker S100 mRNA.

关 键 词：实时定量PCR S100mRNA 骨髓基质细胞 诱导分化 小鼠 

分 类 号：Q813.11[生物学—生物工程]