HPLC测定强力枇杷露中罂粟碱的含量  

Determination of papaverine in Qiangli Pipalu by HPLC

作　　者：秦文杰[1] 马开[2] 高寒[2] 王钢力[3] 林瑞超[3] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]河南省中医药研究院,河南郑州450004 [3]中国药品生物制品检定所,北京100050

出　　处：《中国中药杂志》2006年第16期1330-1332,共3页China Journal of Chinese Materia Medica

摘　　要：目的：建立高效液相色谱法测定强力枇杷露中罂粟碱含量的方法。方法：采用C18色谱柱，乙腈-0．02·mol·L^-1磷酸二氢钠水溶液（含0．2％三乙胺，磷酸调pH3）（25：75）为流动相，流速1mL·min^-1，柱温40℃，检测波长240nm。结果：罂粟碱在0．0201-0．1005μg质量与峰面积积分值成良好的线性关系，平均回收率99．1％，RSD2．3％。结论：该法简便、准确，可作为强力枇杷露的含量测定方法。Objective： To establish a method for the determination of the papaverine content in Qiangli Pibalu by HPLC. Method： A C18 column with a solvent system of acetonitrile-0.02 mol·L^-1 sodium dihydrogen phosphate （0.2% triethylamine, phosphoric acid, at pH 3） （25：75） and UV detection 240 nm were used. The flow rate was 1.0 mL·min^-1. The column temperature was maintained at 40℃. Result： There was a good linear relationship between the absorption value and the concentration in the range of 0.020 2-0. 100 5μg for papaverine. The average recovery rates were 99.1% （RSD 2.3%）. Conclusion： The method is simple, accurate and can be used to determine the contents in Qiangli Pibalu.

关 键 词：强力枇杷露 高效液相色谱法 罂粟碱 

分 类 号：R286[医药卫生—中药学]