五苓散对阿霉素肾病大鼠肾脏血流动力学的影响  

Alteration of renal hemodynamic in adriamycin-induced nephrosis rats administrated with Wulingsan

作　　者：何岚 蔡宇[2] 陈朝晖[1] 蒋建敏[3] 王宁生[1] 

机构地区：[1]广州中医药大学临床药理研究所,广东广州510405 [2]暨南大学药学院,广东广州510632 [3]中山大学药学院,广东广州510080

出　　处：《中国中药杂志》2006年第16期1358-1360,共3页China Journal of Chinese Materia Medica

基　　金：广东省中医药管理局项目(1040063)

摘　　要：目的:了解五苓散是否改善阿霉素(ADR)肾病大鼠肾脏局部血流动力学指标。方法:制备ADR肾病大鼠动物模型,灌胃给予五苓散4周后,测定各组动物的菊糖及对氨基马脲酸钠清除率及肾组织内皮素(ET)、血管紧张素Ⅱ(AngⅡ)、一氧化氮(NO)等相关因素的水平,并计算肾小球滤过率、肾血浆流量及肾血管阻力值。结果:模型组的肾小球滤过率未见明显变化,肾血浆流量显著降低,肾血管阻力值显著升高,肾组织ET,AngⅡ水平显著升高,NO水平显著降低(与正常对照组比较,P<0.05),五苓散可以增加ADR肾病大鼠的肾血浆流量,降低其肾血管阻力值,降低其肾组织ET,AngⅡ水平(与模型对照组比较,P<0.05)。结论:五苓散可以改善ADR肾病大鼠的肾组织局部的血流动力,这一作用与其影响了肾组织局部相关血管活性因子的水平有关。Objective： To investigate the effect of traditional classical compound Wulingsan on renal hemodynamic in rats with adriamycin（ADR）-induced nephrosis. Method： After establishing a model of rats with adriamycin-induced nephrosis, we administrated wulin-san to the ADR rats via oral gavage for four weeks and measured mean arterial blood preasure（MABP） with manometer. Renal clearance of paraaminohippuric acid （PAH）and inulin were detected, then renal plasma flow（RPF） and glomenular filtration rate（GFR） were calculated. Renal vascular resistance（RVR） was calculated as the division of MABP by RPF. Renal endothelin （ET） and angioteusin Ⅱ （Ang Ⅱ）were detected with radioimmunity assay kits, and nitrous oxide（NO） was detected with biochemical kits. Result： There was no significant change of GFR in ARD rats, but RPF and NO were decreased, which accompanied by enhanced RVR, ET and AngⅡ . RPF was increased in the administrated rats , in company with RVR, ET and AngⅡ decreased, whereas NO was not influenced after the administration. Conclusion： Wulingsan can improve the renal hemodynamic in ADR rats, at least in part by modulating the levels of vasoactive factor.

关 键 词：五苓散 阿霉素肾病 大鼠 血流动力 

分 类 号：R285.5[医药卫生—中药学]