漆树漆酶催化单宁酸的氧化反应研究  

Study on Catalytic Oxidation of Tannic Acid by Laccase from Rhus Vernicifera

作　　者：蓝虹云[1,2] 雷福厚 蓝柳柳[1] 韩金华[1] 李中华[3] 

机构地区：[1]广西民族大学化学与生态工程学院 [2]华中师范大学化学学院,湖北武汉430079 [3]华中师范大学化学学院

出　　处：《化学与生物工程》2006年第8期24-26,共3页Chemistry & Bioengineering

基　　金：广西教育厅资助项目

摘　　要：用分光光度法考察漆树漆酶催化单宁酸的氧化情况，探讨了pH值、反应温度、单宁酸浓度、有机酸和金属离子等对催化反应的影响。结果表明，当反应温度为30℃时，漆树漆酶催化反应效果较好；在pH值为4．2～9．8的范围内，反应速率随pH值增大而加快；单宁酸浓度在1．47×10^-6～2．94×10^-1mol·L^-1时，反应速率随底物浓度的增大呈线性增加；Cu^2＋、Mg^2＋和Ni^2＋三种金属离子对催化反应有激活作用，其中Cu^2＋影响最大；而柠檬酸和乙二胺四乙酸对酶催化反应均有抑制作用。The oxidation of tannic acid catalyzed by laccase from Rhus vernicifeka was studied through spectrophotometer. The effects of conditions such as pH value, temperature, concentration of tannic acid, organic acid and metal ions on reaction were discussed. The experimental results showed that the reaction rate rose while the pH value was rising at the range of 4.2-9. 8,and the optimum temperature was 30℃. With the increase of concentration of tannic acid from 1.47× 10 ^-5 mol .L^-1 to 2.94× 10^-1 mol.L^-1 , the rate of oxidation went up as well. The metal ions Cu^2＋ , Mg^2＋ and Ni^2＋ could activate the catalytic reaction, especially the Cu^2＋. The catalytic oxidation was restrained by citric acid or ethylene diaminetetraacetic acid.

关 键 词：漆树漆酶 单宁酸 催化氧化 

分 类 号：O629.8[理学—有机化学]