丙酮酸脱氢酶测活方法比较研究  

Comparison of Measuring Methods for Activity of Pyruvate Dehydrogenase

作　　者：何亚辉[1] 姜英[1] 袁均林[1] 贺红武[2] 谭宏亮[1] 杨旭[1] 丁书茂[1] 

机构地区：[1]华中师范大学生命科学学院,湖北武汉430079 [2]华中师范大学化学学院,湖北武汉430079

出　　处：《化学与生物工程》2006年第8期27-30,共4页Chemistry & Bioengineering

基　　金：国家973计划资助项目(2003CB114406)

摘　　要：对目前几种丙酮酸脱氢酶测活的方法进行了比较研究。结果表明,在测定丙酮酸脱氢酶活性时,2,6-二氯吲哚酚法灵敏度较高,是一种经济有效的好方法,铁氰化钾法不论从反应时间和现象变化上看都是最差的。但2,6-二氯吲哚酚法不能用于测定丙酮酸脱氢酶复合体活性,这时用辅酶A法较好,其灵敏度也比较高。Several current measuring methods for activity of pyruvate dehydrogenase are compared and studled. The results show that 2,6-DCPIP method is economic and effective with relatively high sensitivity for measuring activity of pyruvate dehydrogenase （El）. The potassium ferricyanide method is the worst as to reaction time and change of phenomena. But 2,6-DCPIP can＇t be used for measuring activity of pyruvate dehydrogenase complex,whereas CoA method is suitable with good sensitivity.

关 键 词：丙酮酸脱氢酶复合体 丙酮酸脱氢酶 活性 测活方法 

分 类 号：Q55[生物学—生物化学]