邻二氮菲-铁(Ⅲ)测定半胱氨酸  

Determining Cysteine with o-phenanthroline-Fe^(3+)

作　　者：冯世龙[1] 王红庆[1] 

机构地区：[1]信阳农业高等专科学校,河南信阳464000

出　　处：《信阳农业高等专科学校学报》2006年第3期131-132,共2页Journal of Xinyang Agricultural College

摘　　要：研究了邻二氮菲-Fe3+与半胱氮酸的反应,发现半胱氨酸浓度在1.0×10-5~1.8×10-4mol.L-1范围内其产物吸光度值符合朗伯比耳定律。检测限1.0×10-6mol.L-1,表观摩尔吸光系数7.9×103L.mol-1.cm-1。采用本法测定谷氨酸、赖氨酸、半胱氨酸混合试样中半胱氨酸含量,相对标准偏差≤2%,结果令人满意。The article has researched the reaction of mixing o-phenanthroline and cysteine up. It has been found that absorbanee of the production is in line with Beris low when the concentration of eysteine varies from 1.0 × 10 ^-5 - 1.8 × 10^ -4mol · L^-1. The minimum concentration detected is 1.0 × 10^-6mol · L^-1. To determine the eysteine Glutamie aerd Lysine o-phenanthroline and eysteine with this method, the relative meansquare deviation is equal to 2% or less than it. The result is satisfying.

关 键 词：邻二氮菲-Fe^3+ 光度法 半胱氨酸 

分 类 号：O657.32[理学—分析化学]