硬脑膜细胞体外培养模型的建立  

THE CONSTRUCTION OF AN IN VITRO MODEL OF DURAL CELLS

作　　者：周峰[1] 陈高[1] 张建民[1] 

机构地区：[1]浙江大学医学院附属第二医院神经外科,浙江杭州310009

出　　处：《中国应用生理学杂志》2006年第3期371-374,共4页Chinese Journal of Applied Physiology

摘　　要：目的：建立兔硬脑膜缺损的体外培养模型，观察不同细胞外基质对其愈合过程的影响，研究硬脑膜修复的机制，为以后检测硬脑膜移植物提供理论基础。方法：兔硬脑膜体外培养，再用免疫细胞化学方法和扫描电镜进行观察。结果：预先铺过胶原的培养板中培养8～10d后有细胞从硬脑膜缺损的边缘逸出、增殖，13～15d硬脑膜缺损愈合；而以多聚赖氨酸及层粘连蛋白铺底的培养板中没有看到细胞逸出的现象。硬脑膜细胞对波形蛋白抗体呈强阳性反应，对第Ⅶ因子抗体则为阴性反应；在电镜下可以看到新生胶原的生成。结论：成功建立一种硬脑膜缺损的体外培养模型；成纤维细胞从硬脑膜创缘的逸出、增殖是硬脑膜缺损愈合的重要机制。Aim： To study the heating mechanism of duraplasty a model of rabbit dural healing was constructed in vitro and the influences of collagen , laminin, polylysine on the migration and proliferation of dural cells were compared. MetiNg： Rabbit dura pieces 1.5 cm × 1.5 cm in size were created and were perforated in their central part with a 2 mm punch to mimic a dural defect. The dural pleces were cultured in 24-well plates which had been coated with collagen, laminin and polylysine respectively and the influence of different extracellular matrix on the migration and proliferation of dural cells was observed. Cells were subcultured on slides for immunocytochemistry to identify the charecteristics of dural cells. The dural healing was observed by scanning electronic microscope. Results. Only the dura pieces cultured on collagen coated wells showed migration of cells into the central defect after a period of 8-10 days and dural defect healing occurred after 13 - 15 days. Dural cells stained strongly positive with antibodies against vimentin and negative with Ⅶ factor. New collagen fibers were observed in the dural defects. Conclusion; A kind of cell model for dural healing was constructed successfully in vitro. Cell migration from the dural defect margin is an important mechanism in the process of wound healing after duraplasty.

关 键 词：硬脑膜重建 体外培养 成纤维细胞 细胞外基质 

分 类 号：R651.1[医药卫生—外科学]