知母皂苷B对大鼠海马注射β-AP（25～35）致tau蛋白磷酸化的影响  

Effects of Saponin B from Anemarrhena asphodeloides Bunge on tau hyperphosphorylation induced by beta-amyloid peptide (25-35) in rats

作　　者：钟雷[1] 谭洁[2] 欧阳石[3] 徐江平[3] 

机构地区：[1]广州军区武汉总医院神经内科,湖北武汉430070 [2]解放军第94医院内三科,江西南昌330002 [3]南方医科大学药学院药理学系,广东广州510515

出　　处：《南方医科大学学报》2006年第8期1106-1109,共4页Journal of Southern Medical University

基　　金：国家863计划新药博士基金资助项目(2003AA2Z3535)~~

摘　　要：目的观察大鼠双侧海马注射β淀粉样肽[β-AP(25￣35)]后,海马内p53、Wnt途径抑制剂DKK和磷酸化tau蛋白表达变化,并用此动物模型观察知母皂苷B的作用。方法用RT-PCR法检测p53和DKKmRNA表达的变化,用免疫组化法检测磷酸化的tau蛋白。结果AD模型组海马内神经元的tau蛋白过度磷酸化,而p53和DKKmRNA的表达与假手术对照组相比也显著增多;而知母皂苷B给药治疗组均可使这种现象得到明显改善。结论知母皂苷B可通过抑制β-AP引起的p53及其后续的DKK的表达增加而有效改善AD大鼠脑内神经元的tau蛋白过度磷酸化。Objective To investigate the changes in the expressions of p53, DKK （the inhibitor of Wnt pathway） and phosphorylated tau in rat bilateral hippocampus after β-amyloid peptide （β-AP） （25-35） injection, and observe the effect of saponin B fi＇om A nemarrhena asphodeloides Bunge （SAaB） in this model. Methods After bilateral injection of β-AP （25-35） into the hippocampus of rats, RT-PCR was performed for observing the changes in p53 and DKK mRNA expressions and immunochemistry carried out to detect the changes in phosphorylated tau protein. Results RT-PCR showed increased p53 and DKK mRNA expression and immunochemistry revealed increased phosphorylated tau-positive cells in rat hippocampus after β-AP （25-35） injection, and administration of SAaB significantly ameliorated these changes, Conclusion SAaB can significantly ameliorate β-AP-induced tau hyperphosphorylation by inhibiting increased p53 and DKK mRNA expressions in response to β-AP injection.

关 键 词：知母皂苷B TAU蛋白 P53 DKK Β淀粉样肽 

分 类 号：R285[医药卫生—中药学]