槐果碱体外抗柯萨奇病毒B3m的作用  

Role of Sophocarpine in Coxsackievirus B3m Infection in vitro

作　　者：张星宇[1] 陈曙霞[2] 刘晶星[3] 钱富荣[2] 

机构地区：[1]上海交通大学医学院仁济医院急诊科,上海200127 [2]上海交通大学医学院仁济医院心内科,上海200127 [3]上海交通大学基础医学院微生物学教研室

出　　处：《上海交通大学学报（医学版）》2006年第8期892-894,共3页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的 以HeLa细胞为模型，观察从苦参中提取的槐果碱在体外抗柯萨奇病毒B3m（CVB3m）的作用。方法 ①用微量细胞培养法观察槐果碱对HeLa细胞的毒性。②用细胞病变效应（CPE）抑制法观察槐果碱体外抗CVB3m作用。③用MTT法和结晶紫染色法观察槐果碱对CVB3m感染的HeLa细胞的保护作用：在96孔板上种植HeLa细胞并予CVB3m吸附1h，加入不同浓度的槐果碱，并设立病毒、细胞、槐果碱对照，培养15h后以MTT法和结晶紫染色法测定比较各组细胞能量代谢率和细胞存活数。结果 ①槐果碱稀释到〈391μg／mL，对HeLa细胞无毒性；≥783μg／mL引起HeLa细胞CPE。②槐果碱6．25～50μs／mL有直接抗病毒作用，减轻CPE；〉100μg／mL反而会加重、加快CPE。③槐果碱1．56～25μg／mL对感染CVB3m的HeLa细胞有保护作用，用MTT法和结晶紫染色法测得细胞能量合成代谢、细胞存活数较病毒对照组增加（P〈0．05）；槐果碱在50、100μg／mL时反而加重病毒对细胞的抑制，使细胞存活数、细胞代谢率较病毒对照组下降（P〈0．05）。结论 一定浓度的槐果碱在体外有抗CVB3m作用，对感染CVB3m的HeLa细胞有保护作用。Objective To observe the in vitro anti-Coxsackievirus B3m （ CVB3m ） effect of sophocarpine（ SC ） extracted from Sophora flavescens, a traditional Chinese herb. Methods HeLa cells were cultured and the microdose cytopathic effect （CPE） assays were applied to detect the toxicity of SC. CPE-inhibitory assays were used to observe the in vitro anti-CVB3m effect of SC. MTT and crystal assays were introduced to examine the anti-CVB3m effect of SC. HeLa cells were infected with CVB3m and added with SC in different concentrations 15 h later. The viability and number of survival of HeLa cells were determined by MTT and crystal violet assays, respectively. Results No toxicity was found on HeLa cells by SC with concentrations 〈 391 μg/mL, while CPE was caused with concentrations ≥783 μg/mL. SC with concentrations from 6.25 to 50 μg/mL had direct anti-CVB3m effect and could alleviate the CPE of CVB3m on HeLa cells,While with concentrations 〉 100 μg/mL, SC could accelerate and aggravate the CPE. SC could protect the CVB3m-infected HeLa cells with concentrations from 1.56 to 25μg/mL, and the viability and cell number measured by MTT and crystal violet assay in the SC-handled cells were higher and bigger than those in the virus infected ones. However, the inhibitory effect of virus was exacerbated with higher concentrations （50 and 100μg/mL） , and the cell number and viability of the SC-handled cells were smaller and lower than those of the infected ones. Conclusion SC with a proper concentration has the in vitro anti-CVB3m effect and may protect HeLa cells from CVB3m infection.

关 键 词：槐果碱 柯萨奇病毒B3 HELA细胞 

分 类 号：R285[医药卫生—中药学]