GNB3基因C825T位多态性与肥胖相关性  

Correlation between G-Protein β3 Subunit Gene C825T Site Polymorphism and Obesity

作　　者：楼晓明[1] 朱心强[1] 秦建芬[2] 丁刚强[3] 

机构地区：[1]浙江大学医学院公共卫生系,杭州310006 [2]浙江大学医学院邵逸夫医院 [3]浙江省疾病预防控制中心营养与食品安全研究所

出　　处：《上海交通大学学报（医学版）》2006年第8期895-898,共4页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的建立一种快速检测G蛋白β3亚单位(GNB3)825位基因单个核苷酸多态性(SNP)的方法,分析GNB3825位基因SNP与肥胖的相关性。方法采用实时荧光PCR方法快速检测国内21个省共420个样品的GNB3825位基因SNP,得出该位点的基因型分布频率,并与基因测序法比较检测准确性。207位体检人群分别检测GNB3825位基因SNP、体质量、体质量指数(BMI)和脂肪含量指标,分析GNB3825位基因SNP与肥胖相关指标的关系。结果实时荧光PCR法检测结果显示,国内825T和825C单倍体的分布频率分别为46.90%和53.10%。基因型825TT、825TC和825CC的分布频率分别为22.38%、51.42%和28.10%,未发现其他基因型,其检测结果与基因测序检测结果完全一致。基因型825TT组BMI和脂肪含量显著高于825TC组和825CC组;基因型825TT组体质量指标显著高于825CC组,但与825TC组无统计学差异。结论成功建立快速检测GNB3825位基因SNP的方法;GNB3825位基因型TT可能与肥胖的发生相关。Objective To establish a method for rapidly detecting the G-protein β3 subunit （GNB3） 825 site single nucleotide polymorphism （SNP） and to analyse the relationship between GNB3 825 site gene SNP and obesity. Methods The real-time fluorescent PCR was employed to analyse the GNB3 825 site gene SNP of 420 samples from 21 provinces and the the frequencies of genotypes were compared with those detected by gene sequencing. GNB3 825 site genotype, body weight, body mass index （BMI） and fat content were examined from 207 subjects and the correlation between GNB3 825 site gene SNP and obesity was analysed. Results The result by real-time fluorescent PCR showed that the frequencies of 825T and 825C haploid were 46.90% and 53.10% , respectively, and the frequencies of 825TT, 825TC and 825CC genotype were 22.38% , 51.42% and 28.10% , respectively, with no other genotype detected, which was consistent with the result by gene sequencing. BMI and fat content were significantly higher in subjects with GNB3 825TT than in subjects with other genotypes. Body weight was much higher in subjects with GNB3 825TT genotype than in subjects with 825CC genotype, but not significantly different with 825CT genotype. Conclusion A new rapid method for the detection of GNB3 825 site SNP has been successfully established. There existed significant correlation between GNB3 825TT genotype and obesity.

关 键 词：G蛋白Β3亚单位 单个核苷酸多态性 聚合酶链式反应 肥胖 

分 类 号：Q987[生物学—遗传学]