早期和非早期类风湿关节炎患者外周血CD4^+ T细胞基因表达差异  

Gene expression profile of the peripheral CD4^+ T cells in patients with early and non-early rheumatoid arthritis

作　　者：赵林华[1] 吕诚[1] 肖诚[1,2] 查青林[3] 闫小萍[2] 王建明[2] 张英泽[2] 杨卫彬[1] Youwen Zhou 李梢[5] 吕爱平[1,6] 

机构地区：[1]中国中医研究院基础理论研究所,北京100700 [2]中日友好医院,北京100029 [3]江西中医学院国家工程研究中心,南昌330006 [4]University of British Columbia, Vancouver, Canada [5]清华大学生物信息研究所,北京100084 [6]上海高校中医内科E研究院

出　　处：《现代免疫学》2006年第4期336-339,共4页Current Immunology

基　　金：国家自然科学基金重点资助项目(90209002);上海高校中医内科E-研究院资助项目(E03008)

摘　　要：为了探讨早期、非早期类风湿关节炎(RA)患者与健康人外周血CD4+T细胞的基因表达差异。采集早期、非早期RA患者及健康人空腹静脉血,纯化得到CD4+T淋巴细胞,利用基因芯片检测和分析技术,探索早期、非早期RA患者与健康人外周血CD4+T淋巴细胞基因表达差异。结果:非早期与早期RA患者比较,外周血CD4+T淋巴细胞有83条基因表达存在显著性差异,其中上调3条,下调80条,主要涉及信号转导。与健康人比较,有45条基因异常表达,其中13条基因在RA的早期和非早期较正常对照组表达降低,30条在早期高表达;只有2条与早期与非早期比较差异表达的83条基因重复;差异基因涉及信号转导和免疫应答,以及相关的信号转导途径。早期与非早期RA患者外周血CD4+T淋巴细胞基因表达谱存在明显差异,与健康人比较的差异基因与RA早期和非早期比较的差异基因不一致。To find out the difference in gene expression profile of the peripheral CD4^＋ T cells in patients with early and non-early rheumatoid arthritis （RA）, the CD4^＋ T lymphocytes from peripheral blood of patients with RA and healthy individuals were obtained and then the gene-clip test and analysis techniques were used to detect the differences of the gene expression profiles. It was found that there were 83 gene expression differences detected among the early and non-early progressing cases of RA, in which 3 showed up-regulation and 80 showed down-regulation, mainly referring to signal transduction pathways. Compared with healthy individuals, there were 45 gene expression differences, in which the expression of 13 genes was reduced in early and non-early stages of patients with RA in comparison with those of the healthy controls, 30 genes highly expressed during early stage and 2 genes repeated the differences of the 83 genes among the early and non-early progressing cases of RA, mainly referring to immune responses and signal transduction as well as the related pathways. It is concluded that differences exist in the gene expression profile between the early and non-early stages of patients with RA and the differently exprssed genes mainly refer to signal transduction and its relatd pathways.

关 键 词：基因表达 基因芯片 类风湿性关节炎 

分 类 号：R593.22[医药卫生—内科学]