A novel multi-functional cell-based microphysiometer  

作　　者：XU Ying XU Gaixia LIU Qingjun CAI Hua LI Yan LI Rong WANG Ping 

机构地区：[1]Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China [2]State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai 200050, China

出　　处：《Progress in Natural Science:Materials International》2006年第8期808-816,共9页自然科学进展·国际材料（英文版）

基　　金：Supported by National Natural Science Foundation of China (Grant Nos. 30270387, 30570492)State Key Laboratory of Transducer Technology of China (Grant No. SKT0403)Foundation for the Education Bureau of Zhejiang Province of China (Grant No. 20040197)

摘　　要：This paper presents a novel multi-functional microphysiometer for simultaneous measurements of several extracellular ion concentrations and action potential measurement in living cells based on MLAPS （multi-light addressable potentiometric sensor）. In the microphysiometer, sorts of sensitive membranes are illuminated in parallel with n light sources at working frequencies, and the response amplitudes of each frequency component can be measured on-line by parallel processing algorithm. In the experiments, the relations of the extracellular environmental H^＋ , Na^＋, K^＋ , Ca^2＋ under the effects of western medicines （dilantin, phenobarbital sodium） and Chinese drugs （scutellaria, medlar, hemlock parsley） were analyzed, and the effects of several drugs were evaluated. Moreover, the action potential signals of different cell types （cardiac myocytes and neurons） could he measured and analyzed by LAPS. By detecting these parameters, the system can monitor the real-time process of the cell metabolism and action potential, observe the functional responses of different kinds of membrane-bound receptors, and evaluate the activities of drugs.

关 键 词：multi-functional microphysiometer cell-based biosensor cellular ion concentration measurement cellular action potentiul 

分 类 号：R318[医药卫生—生物医学工程]