利用ISSR标记对新疆白梭梭居群的遗传多样性分析  

Analysis of Genetic Diversity of Haloxylon persicum (Chenopodiaceae) in Xinjiang by ISSR

作　　者：张萍[1] 董玉芝[1] 魏岩[1] 胡成志[2] 

机构地区：[1]新疆农业大学林学院,新疆乌鲁木齐830052 [2]新疆大学,新疆乌鲁木齐830046

出　　处：《云南植物研究》2006年第4期359-362,共4页Acta Botanica Yunnanica

基　　金：国家自然科学基金项目(30260009);新疆维吾尔自治区自然科学基金项目(200421112)

摘　　要：利用ISSR分子标记对新疆白梭梭4个居群，105个个体进行了遗传多样性的比较分析。在供试材料中，11个引物共扩增出171个多态位点，多态位点百分率为84．85％，4个居群的多态位点百分率差异在33．92％．40．35％之间。Shannon多样性指数（I）为0．3518，物种水平的Nei基因多样度（h）为0．3482。遗传变异分析表明，物种水平的居群间遗传分化系数Gst为0．6238，居群间的基因流Nm为0．3016。遗传分析表明吐鲁番居群和甘家湖居群的遗传距离最近。ISSR molecular markers were used to detect genetic diversity of 105 individuals in four populations of Haloxylon petsicure. 171 polymorphic bands were induced by amplication primers. Percentage of polymorphic bands is 84.85% and the difference among four populations ranged from 33.92% to 40.35 %. Shannon＇s indices of diversity （I） is 0.3518 and Nei＇s gene diversity （h） is 0.3482 respectively. Genetic variance analysis showed that the coefficient of gene differentiation in the species is 0.6238 and the gene flow is 0.3016 among populations. Genetic distance and cluster analysis indicated that the populations of Ganjiahu and Tulufan had the smallest genetic difference.

关 键 词：白梭梭 ISSR 遗传多样性 

分 类 号：Q943[生物学—植物学]